Fluorescence Activated Cell Sorter

描述(申请人提供)：流式细胞术及其扩展细胞分选的应用已经彻底改变了生物医学研究的许多方面。随着单抗的发明和使用，可以识别细胞表面分子，从而识别细胞亚群，细胞分选允许通过重建方案分离具有特定表型的细胞，用于体外和体内的功能研究。随着转化医学领域和靶向治疗的发展，能够从参与临床试验的患者中分离出相关的细胞亚群，以便对此类治疗和制剂进行改进是至关重要的。UMDNJ-Robert Wood Johnson医学院和新泽西州立大学罗格斯大学的26个由NIH支持的研究小组正在请求BD FACSAria细胞分选器。所选的仪器将提供通过高速分选分离稀有细胞群的关键能力。作为这些机构一系列临床研究和试验的一部分，对计划中的研究也至关重要的是对新鲜人类细胞进行分类的能力。因此，该仪器需要适当的(BL2)生物安全容器。虽然大多数用户以前曾在他们的研究中使用过流式细胞仪和细胞分类，但所要求的仪器将第一次在我们的机构允许上述能力，从而扩大用户的研究范围。公共卫生相关性：我们正在申请共享仪器赠款，用于购买BD FACSARIA II高速细胞分选机，该分选机能够对人类细胞群体进行分选，以此作为制定预防、诊断和治疗癌症和其他由基因和传染病引起的疾病并研究这些疾病的潜在生物学的手段。这些研究将由我们的教员和新泽西州立大学罗格斯大学的教员在UMDNJ-Robert Wood Johnson医学院的校园内进行。

DESCRIPTION (provided by applicant): The application of flow cytometry and its extension cell sorting has revolutionized many aspects of biomedical research. Paired with the invention and use of monoclonal antibodies which has allowed the identification of cell surface molecules and thus the identification of cell subsets, cell sorting has allowed the isolation of cells with a particular phenotype for use in functional studies both in-vitro and in-vivo through reconstitution protocols. As the field of translational medicine and the development of targeted therapeutics progresses, it is critical to be able to isolate relevant cell subsets from patients taking part in clinical trials to allow the refinement of such treatments and agents. A group of 26 NIH-supported research groups at UMDNJ-Robert Wood Johnson Medical School and Rutgers, The State University of New Jersey are requesting a BD FACSAria cell sorter. The instrument chosen will provide the critical capability of isolating rare cell populations via high speed sorting. Also critical to the planned studies is the ability to sort fresh human cells as part of a series of clinical studies and trials at the institutions. Thus, the instrument requires appropriate (BL2) Biosafety containment. While the majority of the users have previously used flow cytometry and cell sorting in their studies, the requested instrument will, for the first time at our institutions, allow the above capabilities and thus the expansion of the scope of users' studies. PUBLIC HEALTH RELEVANCE: We are requesting a Shared Instrumentation Grant award for the purchase of a BD FACSARIA II high speed cell sorter with the capability of sorting human cell populations as a means of developing strategies to prevent, diagnose, and treat cancer and other genetically and infectious agent caused diseases and to study the underlying biology of these conditions. These studies will be carried out on the campus of the UMDNJ-Robert Wood Johnson Medical School by our faculty and that of Rutgers, The State University of New Jersey.