IN VIVO CHARACTERIZATION OF RNA VIRUS ASSEMBLY LINES WITH EM TOMOGRAPH

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 病毒是世界各地许多人类疾病的原因，并给社会造成重大经济损失。在美国，流感是一种人类病原体，每年造成的直接和间接成本估计为100亿美元，未来一场严重的大流行的成本可能高达数千亿美元。研究病毒的组装和进入将有助于研究人员开发新的抗病毒靶点和疫苗。这项建议的重点是使用电子显微镜断层扫描来回答关于RNA病毒在体内的生命周期的关键问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Viruses are the cause of many human diseases through out the world, and result in major financial losses to society. In the United States Influenza, a human pathogen, is responsible for an estimated total direct and indirect cost of $10 billion per year, and the costs of a serious future pandemic could cost hundreds of billions of dollars. Investigating virus assembly and entry will help researchers develop novel anti-viral targets and vaccines. The focus of this proposal is to use to electron microscope tomography to answer key questions about RNA virus lifecycle in vivo.