IN VIVO CHARACTERIZATION OF RNA VIRUS ASSEMBLY LINES WITH EM TOMOGRAPH

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 病毒是世界上许多人类疾病的原因，并给社会造成重大经济损失。在美国，流感这种人类病原体每年造成的直接和间接损失估计达100亿美元，而未来一场严重的大流行病的成本可能高达数千亿美元。研究病毒组装和进入将有助于研究人员开发新的抗病毒靶点和疫苗。该提案的重点是使用电子显微镜断层扫描来回答有关RNA病毒在体内生命周期的关键问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Viruses are the cause of many human diseases through out the world, and result in major financial losses to society. In the United States Influenza, a human pathogen, is responsible for an estimated total direct and indirect cost of $10 billion per year, and the costs of a serious future pandemic could cost hundreds of billions of dollars. Investigating virus assembly and entry will help researchers develop novel anti-viral targets and vaccines. The focus of this proposal is to use to electron microscope tomography to answer key questions about RNA virus lifecycle in vivo.