JACK JOHNSON PRT-PRELIMINARY DATA COLLECTION ON P22 BACTERIOPHAGE

JACK JOHNSON PRT-P2​​2 噬菌体的初步数据收集

• 批准号: 8362035
• 负责人: John Emil Johnson
• 金额: $ 0.27万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362035
• 关键词: Anodes; Bacteriophage P22; Bacteriophages; Capsid; Complex; DNA; Data Collection; Data Set; Funding; Grant; Head; Maps; Modeling; Morphology; National Center for Research Resources; Principal Investigator; Proteins; Pump; Radiation; Research; Research Infrastructure; Resolution; Resources; Retinal Cone; Shapes; Source; Structure; Tail; Testing; United States National Institutes of Health; cost; cryogenics; electron density; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. P22 head-tail connector (also known as portal proteins) is an oligomeric cone-shaped protein that connects the capsid to the tail of bacteriophage P22. The portal protein is also an integral component of the machinery that pumps DNA into the phage head. Each subunit of the oligomer has approximately 85.9KDa and the oligomer is expected to have a 12-fold rotational symmetry with overall size of ~1.1MDa. We have obtained crystals of the P22 head-tail connector under various conditions and tested cryogenic conditions on a rotating anode x-ray generator. The crystals have an average size of ~150*50*50 ¿m3 and a perfect rectangular morphology. We hope to collect a high resolution data set and to determine the structure of the complex by using a cryo-EM low resolution electron density map as a starting model.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 p22头尾连接器（也称为门户蛋白）是一种寡聚锥形蛋白，可将衣壳连接到细菌噬菌体p22的尾巴。门户蛋白也是将DNA泵入噬菌体头的机械的组成部分。低聚物的每个亚基都有大约85.9kDa，预计寡聚物的旋转对称性为12倍，总尺寸约为1.1mda。我们在各种条件下获得了P22头尾连接器的晶体，并在旋转的阳极X射线发生器上测试了低温条件。晶体的平均大小〜150*50*50€M3和完美的矩形形态。我们希望收集高分辨率数据集，并通过使用低分辨率电子密度图作为起始模型来确定复合物的结构。