HIGH SPEED ODT FOR IMAGING BLOOD FLOW DYNAMICS & TISSUE STRUCTURE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 组织生理学和解剖学的直接可视化为医生诊断和管理疾病提供了重要信息。 用于成像体内组织结构和血流动力学的高空间分辨率非侵入性技术目前还不能用作临床医学中的诊断工具。 这些技术可能对生物医学研究和患者治疗产生重大影响。 本研究的目的是发展一种高速无创光学成像技术，即光学相干层析成像（OCT）和光学多普勒层析成像（ODT），以高空间分辨率（2-10 5 m）对生物组织的组织结构和血流进行成像。 在我们的实验室获得的初步结果已经证明了这种技术的潜力，在一些临床应用中，成像组织结构和监测血流动力学是重要的。 然而，我们目前的OCT/ODT系统存在四个限制：速度、分辨率、穿透深度和斑点噪声。 提出的研究是针对发展的高速，高分辨率，相位分辨OCT/ODT系统成像组织结构和微循环，克服这些限制。 本课题的具体目标是：（1）设计和研制一种高速、高分辨率的相位分辨OCT/ODT系统，用于高散射生物组织中活体组织结构和血流动力学的层析成像，（2）研制用于相位分辨OCT/ODT的信号处理和图像重建的软件和硬件，（3）研制用于高散射生物组织中活体组织结构和血流动力学的OCT/ODT系统，（4）研制用于高散射生物组织中活体组织结构和血流动力学的OCT/ODT系统。（3）使用重建的犬血在体外和使用鸡绒毛尿囊膜（CAM）模型在体内对血流成像，以验证和优化OCT/ODT系统操作和空间分辨率;以及（4）在动物模型和临床研究中证明OCT/ODT如何辅助诊断和治疗皮肤肿瘤和鲜红斑痣胎记，其中成像组织结构和监测血流是重要的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Direct visualization of tissue physiology and anatomy provides important information to the physician for the diagnosis and management of disease. High spatial resolution noninvasive techniques for imaging in vivo tissue structure and blood flow dynamics are currently not available as a diagnostic tool in clinical medicine. Such techniques could have a significant impact for biomedical research and patient treatment. The objective of the proposed research is to develop a high speed noninvasive optical technique, optical coherence tomography (OCT) and optical Doppler tomography (ODT), for imaging in vivo tissue structure and blood flow with high spatial resolution (2-10 5m) in biological tissues. Preliminary results obtained in our laboratory have demonstrated the potential of this technology for a number of clinical applications where imaging tissue structure and monitoring hemodynamics are important. However, there are four limitations in our current OCT/ODT s yste m: speed, resolution, penetration depth and speckle noise. The proposed research is directed toward the development of a high speed, high resolution, phase resolved OCT/ODT system for imaging tissue structure and microcirculation that overcomes these limitations. The specific aims of this proposal are to: (1) design and develop a high speed high resolution phase resolved OCT/ODT system for tomographic imaging of in vivo tissue structure and blood flow dynamics in highly scattering biological tissues; (2) develop signal processing and image reconstruction software and hardware for phase resolved OCT/ODT; (3) image blood flow in vitro using reconstituted canine blood and in vivo using the chick chorioallantoic membrane (CAM) model to verify and optimize OCT/ODT system operation and spatial resolution; and (4) demonstrate in animal models and clinical studies how OCT/ODT can assist in diagnosis and treatment of skin tumors and port wine stain birthmarks where imaging tissue structur e and monitoring blood flow are important.