HIGH SPEED ODT FOR IMAGING BLOOD FLOW DYNAMICS & TISSUE STRUCTURE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 组织生理学和解剖学的直接可视化为医生诊断和治疗疾病提供了重要信息。用于体内组织结构和血流动力学成像的高空间分辨率非侵入性技术目前还不能作为临床医学的诊断工具。这些技术可能会对生物医学研究和患者治疗产生重大影响。这项研究的目的是发展一种高速的无创光学技术，即光学相干断层扫描(OCT)和光学多普勒断层扫描(ODT)，以获得高空间分辨率(2-10 5m)的活体组织结构和血流成像。我们实验室获得的初步结果表明，这项技术在一些临床应用中具有潜力，其中成像组织结构和监测血流动力学是重要的。然而，我们目前的OCT/ODT S系统存在四个限制：速度、分辨率、穿透深度和散斑噪声。这项研究旨在开发一种高速、高分辨率、相位分辨率的OCT/ODT系统，用于组织结构和微循环的成像，以克服这些限制。该方案的具体目标是：(1)设计和开发高速高分辨率相分辨OCT/ODT系统，用于高散射生物组织中体内组织结构和血流动力学的层析成像；(2)开发相分辨OCT/ODT的信号处理和图像重建软硬件；(3)利用重组犬血和体内鸡绒毛膜尿囊膜(CAM)模型，验证和优化OCT/ODT系统的操作和空间分辨率；以及(4)在动物模型和临床研究中演示OCT/ODT如何在皮肤肿瘤和葡萄酒渍胎记的诊断和治疗中发挥辅助作用，其中成像组织结构和监测血流非常重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Direct visualization of tissue physiology and anatomy provides important information to the physician for the diagnosis and management of disease. High spatial resolution noninvasive techniques for imaging in vivo tissue structure and blood flow dynamics are currently not available as a diagnostic tool in clinical medicine. Such techniques could have a significant impact for biomedical research and patient treatment. The objective of the proposed research is to develop a high speed noninvasive optical technique, optical coherence tomography (OCT) and optical Doppler tomography (ODT), for imaging in vivo tissue structure and blood flow with high spatial resolution (2-10 5m) in biological tissues. Preliminary results obtained in our laboratory have demonstrated the potential of this technology for a number of clinical applications where imaging tissue structure and monitoring hemodynamics are important. However, there are four limitations in our current OCT/ODT s yste m: speed, resolution, penetration depth and speckle noise. The proposed research is directed toward the development of a high speed, high resolution, phase resolved OCT/ODT system for imaging tissue structure and microcirculation that overcomes these limitations. The specific aims of this proposal are to: (1) design and develop a high speed high resolution phase resolved OCT/ODT system for tomographic imaging of in vivo tissue structure and blood flow dynamics in highly scattering biological tissues; (2) develop signal processing and image reconstruction software and hardware for phase resolved OCT/ODT; (3) image blood flow in vitro using reconstituted canine blood and in vivo using the chick chorioallantoic membrane (CAM) model to verify and optimize OCT/ODT system operation and spatial resolution; and (4) demonstrate in animal models and clinical studies how OCT/ODT can assist in diagnosis and treatment of skin tumors and port wine stain birthmarks where imaging tissue structur e and monitoring blood flow are important.