ESTABLISHMENT OF SPECIFIC PATHOGEN FREE RHESUS AND PIGTA: AIDS ANIMAL MODEL

描述（由申请人提供）：预计对印度恒河猴用于艾滋病相关研究的需求继续超过可用性。鉴于非人类灵长类动物用于艾滋病相关的机会性感染研究的增加，以及它们预计用于研究新出现的疾病和可用于生物恐怖主义的物质，这种短缺可能会持续数年。虽然来源国是未开发生产的潜在重要储备，但在国际和国内运输非人类灵长类动物的难度日益增加，这表明依赖进口可能只能提供短期缓解。此外，具有许多研究模型所需的遗传和微生物特征水平的非人灵长类动物目前还无法从来源国生产资源中获得。国内育种计划设法生产出尖端研究所必需的具有良好特征的动物，为目前用于艾滋病疫苗和发病机制研究的印度恒河猴短缺提供了最佳的长期解决方案。对国内育种项目的投资也将确保未来的可用性。该申请的综合目标是提高ONPRC艾滋病研究扩展SPF繁殖群体的能力，以提供具有遗传特征的印度血统恒河猴，使其不受大量地方性和人畜共患病原体的影响，从而提高该资源对前沿机会性病原体和疫苗研究的有用性。实现这一目标的具体目标是：a)维持并继续扩大ONPRC艾滋病研究扩展SPF恒河猴繁殖群，使其达到100只成年雌性繁殖者的目标种群，每年能够生产35只剩余动物用于研究；b)通过确保SPF状态和管理种群以生产已知亲缘关系和遗传背景的mhc定义的后代，优化ONPRC艾滋病研究扩展SPF繁殖群体中生产的恒河猴在疫苗研究中的潜在用途。c)改善必要的基础设施，以维持ONPRC艾滋病研究扩展SPF恒河猴繁殖群体作为封闭和受保护的资源。

DESCRIPTION (provided by applicant): The projected need for Indian rhesus macaques for AIDS-related research continues to exceed availability. Given the increase in nonhuman primate use for AIDS-related opportunistic infection research, and their projected use for research focused on emerging disease and agents exploitable for bioterrorism, this shortage will likely continue for years to come. While source countries are a potentially important reserve of untapped production, the increasing difficulty of transporting nonhuman primates both internationally and domestically suggests relying on imports may provide only short term relief. Further, nonhuman primates with the level of genetic and microbial characterization prerequisite for many research models are currently not available from source country production resources. Domestic breeding programs managed to produce the well characterized animals necessary for cutting-edge research offer the best long-term solution for the current shortage of Indian-origin rhesus macaques for AIDS vaccine and pathogenesis studies. Investment in domestic breeding programs will also insure future availability. The comprehensive objective of this application is to increase the capacity of the ONPRC AIDS Research Expanded SPF Breeding Colony to provide genetically characterized Indian-origin rhesus macaques free of a broad number of enzootic and zoonotic agents to enhance the usefulness of the resource for cutting edge opportunistic agent and vaccine research. The specific aims for accomplishing this objective are to a) maintain and continue to expand the ONPRC AIDS Research Expanded SPF Rhesus Macaque Breeding Colony to a target population of 100adult breeder females capable of producing 35 surplus animals annually for research use, b) optimize the potential usefulness of rhesus macaques produced in the ONPRC AIDS Research Expanded SPF Breeding Colony for vaccine research by ensuring SPF status and managing the colony for the production of offspring of known parentage and genetic background that are MHC-defined, and c) improve infrastructure necessary to maintain the ONPRC AIDS Research Expanded SPF Rhesus Macaque Breeding Colony as a closed and protected resource.