RHESUS SPF COLONY FOR NON AIDS RESEARCH

用于非艾滋病研究的 SPF 恒河猴群体

• 批准号: 8358054
• 负责人: RUDOLF Paul BOHM
• 金额: $ 3.72万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8358054
• 关键词: Acquired Immunodeficiency Syndrome; Animal Experimentation; Animal Testing; Animals; Behavioral; Blood; Bone Marrow; Breeding; Chinese People; Data; Feces; Funding; Grant; Housing; Infection; Macaca mulatta; Methods; National Center for Research Resources; Primates; Principal Investigator; Production; Research; Research Infrastructure; Research Personnel; Research Support; Resources; SIV; Saliva; Screening procedure; Simian T-lymphotropic virus 1; Source; Specific Pathogen Frees; Tissues; United States National Institutes of Health; Viral; Virus; base; cost; germ free condition; minimally invasive; nonhuman primate; occupational health/safety; peer; programs; research study; social group

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Background: The presence of retroviral infection in nonhuman primate research animals makes them unsuitable for a number of research studies. In addition, the presence of B-virus in nonhuman primates used in research is a significant occupational safety and health concern. The specific pathogen free (SPF) program was created to provide rhesus monkeys seronegative for SIV, SRV, STLV-1, and B-virus. The majority of the animals assigned to the SPF breeding program are in the NCRR/OAR AIDS colony which limits assignment of animals to AIDS research programs. The base grant supported SPF colony described here allows allocation of SPF animals to other than AIDS studies. Methods: Yearling rhesus are housed with peers from their natal groups while viral screening occurs during years 1-3 and are then moved into larger breeding groups. Animals testing positive for viral agents are removed from the SPF program. Yearling rhesus macaques are currently being removed from the conventional breeding colony for inclusion in the SPF program. Results/Discussion: The colony currently consists of 1027 animals (298 Chinese-origin rhesus and 729 Indian-origin rhesus). In 2010, a total of 145 animals were made available for assignment to core and affiliate researchers. In addition, piggybacked use of animals in the breeding colony occurred. Tissues, including blood, feces, saliva, and bone marrow, were provided to investigators to support their research programs. Behavioral/observational data were also collected to support research and management programs. All piggyback use of animals is noninvasive or minimally invasive and allows animals to remain in their social groups with no impact to production.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 背景：在非人灵长类研究动物中存在逆转录病毒感染，使得它们不适合用于许多研究。 此外，在研究中使用的非人灵长类动物中存在B病毒是一个重要的职业安全和健康问题。 创建了无特定病原体（SPF）程序，以提供SIV、SRV、STLV-1和B病毒血清阴性的恒河猴。分配给SPF育种计划的大多数动物都在NCRR/OAR AIDS群体中，这限制了将动物分配给AIDS研究计划。这里所描述的基本补助金支持的SPF群体允许将SPF动物分配给艾滋病研究以外的研究。研究方法：一岁的恒河猴与纳塔尔群体的同龄人一起饲养，而病毒筛查在1-3年期间进行，然后转移到更大的繁殖群体。 将病毒因子检测呈阳性的动物从SPF程序中删除。一岁的恒河猴目前正在从传统的繁殖群中移除，以纳入SPF计划。结果/讨论：殖民地目前由1027只动物组成（298只中国猕猴和729只印度猕猴）。 2010年，共有145只动物可分配给核心和附属研究人员。此外，在繁殖群中发生了背驮使用动物的情况。 向研究者提供组织，包括血液、粪便、唾液和骨髓，以支持他们的研究计划。 还收集了行为/观察数据，以支持研究和管理计划。所有背驮动物的使用都是无创或微创的，并允许动物留在它们的社会群体中，而不会影响生产。