Mount Sinai CHEAR Network Laboratory Hub

 描述（由申请人提供）：西奈山伊坎医学院将利用我们在儿童环境健康方面的研究专业知识和弗兰克·劳滕贝格环境健康科学实验室，形成一个新的儿童健康暴露分析资源（CHEARS）实验室网络中心。我们将测量怀孕和儿童时期的环境暴露，包括有针对性和无针对性的，以帮助NIH资助的研究人员确定环境如何影响儿童健康，发育和整个生命周期的疾病风险。我们的团队将开发新的方法来客观地重建过去的化学暴露，使研究人员能够研究与儿童健康相关的易感性窗口。为实现这些目标，将建立四个实验室资源/核心：1）有针对性的分析资源，将分析常见的环境暴露生物标志物（金属、农药、阻燃剂、干扰内分泌的化学品、烟草代谢物、维生素、营养状况、矿物质和其他有机化合物）; 2）使用先进技术（如飞行时间质谱法）的非靶向分析资源，以发现与儿童健康和疾病相关的化学物质，代谢物和其他生物标志物; 3）生物反应指标资源，将环境暴露与免疫功能、表观基因组标记、基因表达、非编码RNA和其他反应生物标志物的变化联系起来; 4）我们将建立一个开发核心，创造新的方法来测量新生物基质中当前和过去的化学暴露（例如牙齿、胎盘），开发新的分析方法来研究新出现的有毒物质，并开发新的方法来评估身体对接触的反应。将建立一个行政核心，以协调计划并与CHEMICAL协调中心、CHEMICAL数据中心和其他CHEMICAL网络实验室中心进行外部沟通。在内部，管理核心将整合我们的资源/核心之间的工作，以简化和优先考虑工作订单，评估检测需求，在开发新检测时推广和传播新检测，协调方案和QA/QC程序，并协调日常运营。该实验室中心将就样本要求、样本质量、结果解释、样本收集、储存方案和样本运输规范向申请人提供建议。实验室中心生成的数据的分析和解释将与数据中心合作提供支持;如果需要，我们将与具有分析能力/专业知识的外部实验室联系，这些实验室不在我们的实验室中心。最后，我谨指出， 该提案将经验丰富的环境卫生科学家与儿科医生、毒理学家、压力研究人员、化学家、接触科学家、流行病学家、计算机科学家、免疫学家和表观遗传学家联系起来，以建立客观衡量儿童环境的基础设施和能力。

 DESCRIPTION (provided by applicant): The Icahn School of Medicine at Mount Sinai will leverage our research expertise in children's environmental health and the Frank Lautenberg Laboratory for Environmental Health Sciences to form a new Children's Health Exposure Analysis Resource (CHEAR) Laboratory Network Hub. We will measure environmental exposures, both targeted and untargeted, across pregnancy and childhood to help NIH-funded researchers determine how the environment affects child health, development and risk of disease across the life span. Our team will develop new methodologies to objectively reconstruct past chemical exposures, allowing researchers to study susceptibility windows as they relate to child health. Four laboratory resources/cores will be established to achieve these goals; 1) a targeted analysis resource that will analyze common environmental exposure biomarkers (metals, pesticides, flame retardants, endocrine disrupting chemicals, tobacco metabolites, vitamins, nutritional status, minerals, and other organic compounds) using state-of-the-art analytical methodologies; 2) an untargeted analysis resource using advanced technologies such as time-of- flight mass spectrometry to discover the chemicals, metabolites and other biomarkers that are associated with child health and disease; 3) a biological response indicators resource that will link environmental exposures to changes in immune function, epigenomic marks, gene expression, noncoding RNA and other response biomarkers; and 4) we will establish a developmental core that will create novel methods to measure current and past chemical exposures in new biological matrices (e.g. teeth, placenta), develop new assays to study emerging toxicants, and develop new methods to assess the body's response to exposure. An Administrative Core will be established to coordinate planning and communication externally with the CHEAR Coordinating Center, the CHEAR Data Center and the other CHEAR Network Laboratory Hubs. Internally, the Administration Core will integrate work among our Resources/Cores to streamline and prioritize job orders, assess assay needs, promote and disseminate new assays as they are developed, harmonize protocols and QA/QC procedures and coordinate day to day operations. This laboratory Hub will advise applicants on sample requirements, sample quality, results interpretation, sample collection, storage protocols and sample shipping specifications. Both analysis and interpretation of Lab Hub-generated data will be supported in cooperation with the Data Center; and if necessary, we will outreach to outside laboratories with analytic capabilities/expertise that do not reside in our Lab Hub. In conclusion, this proposal links highly experienced environmental health scientists with pediatricians, toxicologists, stress researchers, chemists, exposure scientists, epidemiologists, computer scientists, immunologists, and epigeneticists to build the infrastructure and capacity to objectively measure child environments.