Mount Sinai HHEAR Network Targeted Lab Hub

西奈山 HHEAR 网络目标实验室中心

• 批准号: 10875017
• 负责人: Robert O Wright
• 金额: $ 12.96万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10875017
• 关键词: Address; Administrative Supplement; Age; Analytical Chemistry; Automatic Data Processing; Biological; Biological Assay; Biological Markers; Blinded; Chemical Exposure; Chemicals; Chemistry; Child; Chromatography; Client; Clinical Medicine; Clinical Trials; Collaborations; Complex; Counseling; Data; Data Analyses; Data Reporting; Database Management Systems; Disease; Dose; Elderly; Elements; Enrollment; Ensure; Environment; Etiology; Europe; Exposure to; Food; Future; Genes; Genomics; Geographic Locations; Goals; Grant; Hair; Health; Human; Human Development; Human Genome; Informatics; Institution; International; Label; Laboratories; Life Cycle Stages; Liquid substance; Management Information Systems; Mass Spectrum Analysis; Measures; Medicine; Metadata; Metals; Methods; Mexico; Monitor; Occupations; Organic Chemicals; Outcome; Parents; Pattern; Pesticides; Phenotype; Population; Predisposition; Pregnancy; Pregnant Women; Preparation; Procedures; Protocols documentation; Quality Control; Randomized; Reporting; Research; Research Design; Research Personnel; Resources; Risk; Robotics; Sampling; Science; Services; Shapes; Software Design; Specimen; Structure; System; Techniques; Technology; Testing; Time; Tooth structure; Toxicology; U-Series Cooperative Agreements; United States; United States National Institutes of Health; Urine; Woman; Work; autism spectrum disorder; computerized data processing; data centers; data integrity; design; detection limit; early childhood; experience; human error; human population study; innovation; instrument; meetings; method development; multidisciplinary; outreach; pesticide exposure; prenatal; prevent; programs; quality assurance; response; software systems; symposium

Project Summary The Targeted Analysis Resource is a multi-disciplinary, full service exposure biomarker program designed to integrate inorganic and organic environmental measures into existing NIH studies. In this supplement we now extend services to include exposure to pesticides that pose health risk to women and children during critical windows such as pregnancy and early childhood. These pesticides are not readily measured in urine due to short half-lives but we have developed methods to measure them in hair. We hold weekly Executive Steering Committee meetings of the full Lab Hub team to discuss jobs, troubleshoot workflows, and prioritize resources and activities in order to provide state of the art service to HHEAR clients. Our team has considerable expertise in matching the underlying toxicology of targeted exposure biomarkers to the study design, to the biological specimen types collected, and to the health phenotypes measured, in this case hair pesticides. Our lab feasibility assessments help shape the science of a client’s submission towards higher impact studies. We also counsel clients on preventing the use of precious samples on assays that are deemed likely to be below detection limits based on our many years of research experience and understanding of exposure scenarios. We work with the Administrative Core to coordinate services, including sample management, receiving, labeling and tracking of the hair samples. We serve as expert consultants with the Coordinating Center, PI/Clients and other HHEAR Resources. We collaborate with the Data Center in the management of results reporting, ensuring that data are properly formatted and have sufficient metadata, as well as participate in the analysis of exposure/phenotype relationships. We use automated robotic sample handling and preparation methods, high throughput analytical procedures and state of the art inter-laboratory QA/QC procedures which include real time chromatography monitoring and database management through state-of-the-art laboratory information management system (LIMS) software. We also automated data processing to reduce human error. Our methods ensure proper sample tracking and data reporting and avoid mismatches of samples to results. With substantial institutional resources at its disposal, the Targeted Resource has more than doubled the number of mass spectrometry instruments to 16 since our inception, ensuring our capacity to serve the needs of the HHEAR Network. Our team also conducts extensive outreach and is the only HHEAR lab hub to organize an international Exposome Symposia in the US, Europe and Mexico, in collaboration with the Mount Sinai Untargeted Lab Hub. QA/QC protocols and standard operating procedures for all target compounds, including the pesticides that are the focus of this application, have been established to maintain the rigor of our chemical analyses. We developed panels for elements and organic chemicals that in sum assess more than 200 chemicals. We participate in all HHEAR mandated QA/QC and PT programs for metals and organic chemicals to verify our precision and accuracy.

项目摘要 目标分析资源是一个多学科、全方位服务的暴露生物标志物计划，旨在 将无机和有机环境措施纳入现有的NIH研究。在本补充中，我们现在 扩大服务范围，包括在关键时期接触对妇女和儿童构成健康风险的农药 窗口，如怀孕和幼儿期。这些农药在尿液中不易测量， 半衰期很短，但我们已经开发出在头发中测量它们的方法。我们每周举行一次执行指导会议 整个Lab Hub团队的委员会会议，讨论工作、排除工作流故障并确定资源优先级 和活动，以便为HHEAR客户提供最先进的服务。我们的团队有相当多的 将靶向暴露生物标志物的基础毒理学与研究设计相匹配的专业知识， 收集的生物样本类型，以及测量的健康表型，在这种情况下是毛发杀虫剂。我们 实验室可行性评估有助于塑造科学的客户提交对更高的影响研究。我们 还建议客户防止在被认为可能低于标准的检测中使用珍贵样本。 检测限是基于我们多年的研究经验和对暴露场景的理解。 我们与行政核心合作，协调服务，包括样品管理，接收， 标记和跟踪头发样本。我们作为协调中心的专家顾问， PI/客户和其他HHEAR资源。我们与数据中心协作管理结果 报告，确保数据格式正确，并有足够的元数据，以及参与 分析暴露/表型关系。我们使用自动化机器人样品处理和制备 方法、高通量分析程序和最先进的实验室间QA/QC程序， 包括通过最先进实验室进行真实的实时色谱监测和数据库管理 信息管理系统（LIMS）软件。我们还自动化数据处理，以减少人为错误。 我们的方法确保正确的样品跟踪和数据报告，并避免样品与结果的不匹配。 由于有大量的机构资源可供支配，目标资源已使2010 - 2011年的 自成立以来，我们将质谱仪的数量增加到16台，确保我们有能力满足需求 HHEAR网络。我们的团队还进行广泛的推广，是唯一的HHEAR实验室中心， 在美国、欧洲和墨西哥组织一次国际博览会专题讨论会， 西奈非目标实验室中心。所有目标化合物的QA/QC方案和标准操作规程， 包括作为本申请重点的杀虫剂，已经建立了严格的 化学分析我们开发了元素和有机化学品的面板， 200化学品我们参与所有HHEAR授权的金属和有机物的QA/QC和PT计划 化学品来验证我们的精度和准确性。

项目成果

Recent advances in simultaneous analysis of bisphenol A and its conjugates in human matrices: Exposure biomarker perspectives.

• DOI: 10.1016/j.scitotenv.2016.07.062
• 发表时间: 2016-12-01
• 期刊: SCIENCE OF THE TOTAL ENVIRONMENT
• 影响因子: 9.8
• 作者: Andra, Syam S.;Austin, Christine;Yang, Juan;Patel, Dhavalkumar;Arora, Manish
• 通讯作者: Arora, Manish

Racial/ethnic and neighborhood disparities in metals exposure during pregnancy in the Northeastern United States.

• DOI: 10.1016/j.scitotenv.2022.153249
• 发表时间: 2022-05-10
• 期刊: The Science of the total environment
• 作者: Geron M;Cowell W;Amarasiriwardena C;Andra SS;Carroll K;Kloog I;Wright RO;Wright RJ
• 通讯作者: Wright RJ

The Metabolome: a Key Measure for Exposome Research in Epidemiology

• DOI: 10.1007/s40471-019-00187-4
• 发表时间: 2019-06-01
• 期刊: CURRENT EPIDEMIOLOGY REPORTS
• 影响因子: 3.3
• 作者: Walker, Douglas I.;Valvi, Damaskini;Jones, Dean P.
• 通讯作者: Jones, Dean P.

Hormones and women's respiratory health across the lifespan: Windows of opportunity for advancing research.

激素和女性整个生命周期的呼吸健康：推进研究的机会之窗。

• DOI: 10.1016/j.jaci.2021.02.033
• 发表时间: 2021
• 期刊: The Journal of allergy and clinical immunology
• 作者: Wright,RosalindJ

Validated single urinary assay designed for exposomic multi-class biomarkers of common environmental exposures.

• DOI: 10.1007/s00216-022-04159-4
• 发表时间: 2022-08
• 期刊: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
• 影响因子: 4.3
• 作者: Jagani, Ravikumar;Pulivarthi, Divya;Patel, Dhavalkumar;Wright, Rosalind J.;Wright, Robert O.;Arora, Manish;Wolff, Mary S.;Andra, Syam S.
• 通讯作者: Andra, Syam S.