Ultra low temperature freezer

超低温冷冻柜

• 批准号: 344843-2007
• 负责人: Nagy, Eva
• 金额: $ 0.99万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326187
• 关键词: Ultra; low; temperature; freezer

With the renewed fears of an avian influenza outbreak resulting in another human pandemic, it is becoming increasingly important to understand how avian viruses interact with their host, are transmitted and how they might change. Equally important, from the avian point of view, is to develop procedures and vaccines and diagnostic reagents which can help reduce the incidence and spread of viral diseases, including influenza viruses. My research work is on a group of viruses known as avian adenoviruses. Some adenoviruses within this group cause disease in chicken, while other, like the one I work with, are asymptomatic with no effect on chicken health. The virus I chose to work with is called fowl adenovirus 9 (FAdV-9) which does not cause disease but could be an excellent vector to generate vaccines against other avian microbial infectious diseases like influenza. As part of this research, I generate numerous variants of FAdV-9 to test the possibility of using this virus as a vaccine against a variety of infectious diseases. In order to generate these variants, I need to isolate and clone (usually in bacteria) different genes from a variety of microbes before inserting some into my recombinant FAdV-9 vaccine. All of these also require a number of temperature sensitive molecular reagents such as enzymes. Moreover, while testing the various modified FAdV-9 vaccines initially in chickens, I collect many samples (e.g. blood and tissue and fecal samples) to test for the presence of the virus and to study the immune response. It is critical that all these virus variants, cloning intermediates, molecular reagents and animal tissues be stored at very low temperature to preserve their activity for future analysis. In many cases it is important to restudy some material and for that a long term, ultra cold, storage is the best way to preserve the samples. I am therefore requesting funds for an ultra low temperature freezer with a low enough temperature to ensure long term storage and of sufficient size to store the many biological samples I work with and need throughout my research on avian viruses and vaccines.

随着人们对禽流感爆发导致另一场人类大流行的担忧重新抬头，了解禽流感病毒如何与宿主相互作用、传播以及它们如何变化变得越来越重要。从禽类的角度来看，同样重要的是开发程序、疫苗和诊断试剂，以帮助减少包括流感病毒在内的病毒性疾病的发病率和传播。我的研究工作是关于一组被称为禽腺病毒的病毒。这一组中的一些腺病毒会引起鸡的疾病，而另一些，像我所研究的那种，是无症状的，对鸡的健康没有影响。我选择的病毒被称为禽腺病毒9（FAdV-9），它不会引起疾病，但可能是一个很好的载体，用于生产针对其他禽微生物传染病（如流感）的疫苗。作为这项研究的一部分，我产生了FAdV-9的许多变体，以测试使用这种病毒作为针对各种传染病的疫苗的可能性。为了产生这些变体，我需要从各种微生物中分离和克隆（通常在细菌中）不同的基因，然后将一些基因插入我的重组FAdV-9疫苗中。所有这些还需要许多温度敏感的分子试剂，如酶。此外，在最初在鸡中测试各种修饰的FAdV-9疫苗时，我收集了许多样品（例如血液和组织以及粪便样品）来测试病毒的存在并研究免疫应答。至关重要的是，所有这些病毒变体、克隆中间体、分子试剂和动物组织都应在极低温度下储存，以保持其活性，供将来分析使用。在许多情况下，重新研究一些材料是很重要的，因此，长期的超低温储存是保存样品的最佳方法。因此，我申请资金用于超低温冷冻箱，其温度足够低，以确保长期储存，并具有足够的大小来储存我在研究禽流感病毒和疫苗期间使用和需要的许多生物样品。