Upgrade of the confocal microscope for Concordia University

康科迪亚大学共焦显微镜升级

• 批准号: 360101-2008
• 负责人: Sacher, Michael
• 金额: $ 2.6万
• 依托单位: Concordia University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=364146
• 关键词: Upgrade; confocal; microscope; Concordia; University

Researchers at Concordia University rely upon a single confocal microscope for high resolution microscopy of biological samples. This microscope, a Leica TCS SP2 purchased in February 2000, cannot handle some commonly used fluorophores such as wild type green fluorescent protein and enhanced yellow fluorescent protein. In its present configuration this microscope cannot meet the needs of the faculty, especially for multifluorophore labeling with three colours or more, and is thus greatly slowing down productivity. Finally, the outdated CPU controlling the system has become a bottleneck for most basic data processing applications, particulary for 3-D reconstruction, animation and volume reconstruction. The proposed upgrades will allow for additional cell biological experiments, not currently practical, to be performed and will enhance the overall infrastructure at the university, facilitating the needs of the current faculty and making the university a more attractive option for recruiting world-class faculty in the future.

Concordia大学的研究人员依靠单个共焦显微镜对生物样品进行高分辨率显微镜检查。该显微镜是2000年2月购买的Leica TCS SP2，不能处理一些常用的荧光团，如野生型绿色荧光蛋白和增强型黄色荧光蛋白。在其目前的配置，这种显微镜不能满足教师的需要，特别是与三种颜色或更多的多荧光标记，从而大大降低生产率。最后，过时的CPU控制系统已成为大多数基本数据处理应用程序的瓶颈，特别是对于三维重建，动画和体积重建。拟议的升级将允许进行目前不切实际的额外细胞生物学实验，并将加强大学的整体基础设施，促进现有教师的需求，使大学成为未来招聘世界级教师的更具吸引力的选择。