The RgpA-Kgp proteinase-adhesin complex and virulence of Porphyromonas gingivalis

RgpA-Kgp蛋白酶-粘附素复合物与牙龈卟啉单胞菌的毒力

• 批准号: nhmrc : 209081
• 负责人: A/Pr Neil O'Brien-Simpson
• 金额: $ 35.16万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2002
• 资助国家: 澳大利亚
• 起止时间: 2002-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/rgpa-kgp-proteinase-porphyromonas-gingivalis/100270
• 关键词: RgpA; Kgp; proteinase; adhesin; complex

Periodontitis is a bacterial-associated inflammatory disease of the supporting tissues of the teeth which can result in tooth loss. The disease is a major public health problem with a large economic burden. A bacterium Porphyromonas gingivalis has now been identified as a major causative agent of chronic periodontitis. We have identified a major virulence factor of P. gingivalis. This virulence factor is a complex of proteins, encoded by two genes, and is involved in binding and destruction of host tissue. When used as a vaccine in animal models the protein complex protects against P. gingivalis infection. Animal protective sera recognises a segment of the protein complex involved in binding to host substrates. The aim of this project is to continue this work on the characterisation of this complex and its role in virulence in an approach to ultimately develop a defined vaccine against P. gingivalis based on this protein complex, in particular the sequences involved in binding. The expected outcome of this research is the further biochemical characterisation of the RgpA-Kgp protein complex and its role in virulence as well as development of a defined vaccine prepared using recombinant DNA and chemical synthesis techniques that protects against P. gingivalis infection in animal models of disease.

牙周炎是一种与细菌相关的牙齿支持组织炎症性疾病，可导致牙齿脱落。这种疾病是一个重大的公共卫生问题，带来了巨大的经济负担。牙龈卟啉单胞菌现已被确定为慢性牙周炎的主要病原体。我们已经确定了牙龈假单胞菌的一个主要毒力因子。这种毒力因子是一种蛋白质复合体，由两个基因编码，与宿主组织的结合和破坏有关。当在动物模型中作为疫苗使用时，这种蛋白质复合体可以预防牙龈假单胞菌感染。动物保护性血清识别与宿主底物结合的蛋白质复合体的一段。本项目的目的是继续这项关于该复合体的特征及其在毒力中的作用的工作，以最终基于该蛋白质复合体，特别是与结合有关的序列，开发一种明确的牙龈假单胞菌疫苗。这项研究的预期结果是对RgpA-KGP蛋白复合体的进一步生化特征及其在毒力中的作用，以及开发出一种使用重组DNA和化学合成技术制备的明确疫苗，以在动物疾病模型中预防牙龈假单胞菌感染。