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Polymicrobial pathogenesis in a murine periodontitis model.

小鼠牙周炎模型中的多种微生物发病机制。

基本信息

批准号：
nhmrc : 251708
负责人：
A/Pr Neil O'Brien-Simpson
金额：
$ 15.7万
依托单位：
依托单位国家：
澳大利亚
项目类别：
NHMRC Project Grants
财政年份：
2003
资助国家：
澳大利亚
起止时间：
2003-01-01 至 2005-12-31
项目状态：
已结题

来源：
https://researchdata.edu.au/polymicrobial-pathogenesis-murine-periodontitis-model/97767
关键词：
Polymicrobial pathogenesis murine periodontitis model.

项目摘要

Severe periodontal disease is a chronic inflammatory disease of the tooth's supporting tissues and is associated with specific pathogenic bacteria. The disease has a large economic burden and is a major public health problem. Three bacterial species have recently been shown to be closely associated with advanced periodontal disease in humans. One of these three bacterial species has been shown to produce periodontal disease in a mouse model of disease and the major proteins on the cell surface of this bacterium have been characterized. Further, in this mouse model it has been demonstrated that killed cells of the bacterium or the major surface proteins when used as a vaccine prevent development of disease. The purpose of the current proposal is to extend this work to include all three bacterial species that have been associated with disease in humans. The major surface proteins of all these bacterial species when grown together as high density polymicrobial complexes will be determined. Recent results have shown that in high-density, polymicrobial cultures bacteria communicate using signalling systems which can alter the expression of cell surface proteins associated with disease. The ability of the three bacterial species, when grown in polymicrobial culture, to cause periodontal disease in the mouse model will be determined. This will allow the determination of whether a single species and-or multispecies vaccine is capable of protecting against disease in this model. The significance of the work is that it will increase our understanding of the bacteria associated with periodontal disease and it may lead to the development of an effective vaccine to help prevent disease in humans.

严重牙周病是牙齿支持组织的慢性炎症性疾病，与特定的致病菌有关。这种疾病有很大的经济负担，是一个重大的公共卫生问题。最近有三种细菌被证明与人类晚期牙周病密切相关。这三种细菌中的一种已被证明在疾病的小鼠模型中产生牙周病，并且该细菌细胞表面上的主要蛋白质已被表征。此外，在该小鼠模型中，已经证明，当用作疫苗时，细菌的被杀死的细胞或主要表面蛋白防止疾病的发展。目前提案的目的是将这项工作扩展到包括与人类疾病相关的所有三种细菌。将测定作为高密度多微生物复合物一起生长时所有这些细菌物种的主要表面蛋白。最近的结果表明，在高密度，多微生物培养细菌通信使用信号系统，可以改变与疾病相关的细胞表面蛋白质的表达。将确定当在多微生物培养物中生长时，三种细菌物种在小鼠模型中引起牙周病的能力。这将允许确定单种和/或多种疫苗是否能够在该模型中保护免于疾病。这项工作的意义在于，它将增加我们对与牙周病相关的细菌的了解，并可能导致开发有效的疫苗，以帮助预防人类疾病。