Ultralow temperature freezer for archiving microbial evolution experiments

用于存档微生物进化实验的超低温冰箱

• 批准号: 390343-2010
• 负责人: Wing, Boswell
• 金额: $ 0.89万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443169
• 关键词: Ultralow; temperature; freezer; archiving; microbial

The RTI funding requested here will go to support geologically directed microbial evolution experiments aimed at addressing an apparent contradiction that underlies studies of life on early Earth. That is, how can studies of life on early Earth rely on ancient microbes to metabolize exactly like modern microbes when it is known that microbes can evolve on laboratory timescales? Specifically, we are asking for support for an ultra-low temperature freezer in which a culture archive of the experiments will be maintained. This 'fossil' record will be available for reanimation at any time during the experiment, enabling us to directly compete ancestral microbial populations against their descendants. The equipment supported under this RTI award will be part of a host of research projects, ranging from PhD-level experiments to short-term undergraduate research experiences. In particular, it will become a centerpiece in the McGill Stable Isotope Laboratory's training program for post-doctoral, graduate, and undergraduate trainees under the NSERC Collaborative Research and Training Experience Canadian Astrobiology Training Program.

这里要求的RTI资金将用于支持地质导向的微生物进化实验，旨在解决早期地球生命研究的明显矛盾。 也就是说，当已知微生物可以在实验室时间尺度上进化时，对早期地球生命的研究怎么能依赖于古代微生物来完全像现代微生物一样代谢呢？ 具体而言，我们要求支持一个超低温冰箱，其中将保持实验的文化档案。 这个“化石”记录将在实验过程中的任何时候都可以复活，使我们能够直接与祖先的微生物种群竞争。 该RTI奖项支持的设备将成为一系列研究项目的一部分，从博士水平的实验到短期的本科生研究经验。 特别是，它将成为麦吉尔稳定同位素实验室在NSERC合作研究和培训经验加拿大天体生物学培训计划下的博士后，研究生和本科生培训计划的核心。