Spinning Disc Confocal Laser Microscope

转盘共焦激光显微镜

• 批准号: 458880-2014
• 负责人: Geitmann, Anja
• 金额: $ 10.93万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=542066
• 关键词: Spinning; Disc; Confocal; Laser; Microscope

Microscopical imaging of biological specimens represents a crucial component of a variety of research disciplines ranging from molecular and cell biology to physiology. Spinning disk confocal laser microscopy is used to visualize intracellular structures based on the emission of fluorescence light. This type of microscopy is particularly well suited for imaging rapid intracellular movements of organelles and ions and for visualizing samples with low light intensity - typical features of living cells. The Institut de recherche en biologie végétale (IRBV) is part of the Department of Biological Sciences of the University of Montreal and is equipped with a relatively old, conventional confocal laser scanning microscope, but the researchers using this facility do not have access to the equipment required for the advanced needs associated with live cell imaging. Numerous emerging research projects require high quality, fluorescence based imaging methods and the acquisition of a spinning disk confocal laser microscope would accomplish both - alleviate the pressure on the existing confocal and meet the needs of the more advanced users for high sensitivity and high temporal resolution.

生物标本的显微成像代表了从分子和细胞生物学到生理学等各种研究学科的重要组成部分。旋转盘共聚焦激光显微镜是用来观察细胞内的结构基于荧光的发射。这种类型的显微镜特别适合于成像细胞器和离子的细胞内快速运动，以及在低光强度下观察样品-活细胞的典型特征。生物研究所（IRBV）是蒙特利尔大学生物科学系的一部分，配备了一台相对较旧的传统共聚焦激光扫描显微镜，但使用该设备的研究人员无法获得与活细胞成像相关的先进设备。许多新兴的研究项目需要高质量的、基于荧光的成像方法，而获得旋转盘共聚焦激光显微镜将实现这两个目标——减轻现有共聚焦的压力，满足更高级用户对高灵敏度和高时间分辨率的需求。