Dynamics and control of high-precision high-speed atomic force microscopy for protein analysis

用于蛋白质分析的高精度高速原子力显微镜的动力学和控制

• 批准号: 472180-2014
• 负责人: Shan, Jinjun
• 金额: $ 4.37万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=577909
• 关键词: Dynamics; control; precision; speed; atomic

Advanced Electrophoresis Solutions Ltd. (AES) has been developing scientific instruments and providing technical services for protein analysis since 2010. Their unique CEInfinite instruments, based on Whole Column Imaging Detection (WCID), have been used by clients worldwide for high performance, high throughput, high resolution, and reproducible protein analysis. Atomic force microscope (AFM) is a versatile technique that is being used increasingly in protein analysis. The operational range of high-speed AFM imaging is well suited for characterizing structures at the molecular and cellular levels. Currently, AES is working on integrating its high performance CEInfinite instruments with high-precision high-speed atomic force microscopy (HPHS-AFM). This integration will provide innovative, comprehensive protein analysis. In order to achieve this goal, several key technologies related to atomic force microscopy (AFM) will need to be developed and it is expected that these goals will be achieved through this NSERC Collaborative Research and Development (CRD) project. Piezoelectric actuators are the key elements for nanopositioning in AFMs due to many advantages. However, the inherent nonlinearities, such as hysteresis, creep, drifts due to vibration and temperature changes cause inaccuate positioning and low operation speed. This project aims to provide novel and realistic modeling and control approaches for nonlinearities of piezoelectric actuators. The success of this project will make the integration of HPHS-AFM and CEInfinite possible. It will enhance AES' capability in protein analysis and becomes the main provider of such services for many applications.

Advanced Electrophoresis Solutions Ltd.（AES）自2010年以来一直在开发科学仪器并提供蛋白质分析技术服务。其独特的CEInfinite仪器基于全柱成像检测（WCID），已被全球客户用于高性能、高通量、高分辨率和可重现的蛋白质分析。原子力显微镜（AFM）是一种多功能的技术，在蛋白质分析中的应用越来越广泛。高速AFM成像的操作范围非常适合在分子和细胞水平上表征结构。目前，AES正致力于将其高性能CEInfinite仪器与高精度高速原子力显微镜（HPHS-AFM）集成。这种整合将提供创新的，全面的蛋白质分析。为了实现这一目标，需要开发与原子力显微镜（AFM）相关的几项关键技术，预计这些目标将通过NSERC合作研究与开发（CRD）项目实现。 压电驱动器具有许多优点，是AFM纳米定位的关键元件。然而，由于其固有的非线性，如滞后、蠕变、振动和温度变化引起的漂移，导致定位不准确和操作速度低。本计画旨在提供新颖且实际的压电致动器非线性建模与控制方法。该项目的成功将使HPHS-AFM和CEInfinite的集成成为可能。它将增强AES在蛋白质分析方面的能力，并成为许多应用此类服务的主要提供商。