Development of field deployable technologies for improved health and environmental outcomes

开发可现场部署的技术以改善健康和环境成果

• 批准号: RGPIN-2015-05329
• 负责人: Sullivan, Pierre
• 金额: $ 1.6万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=590366
• 关键词: Development; field; deployable; technologies; improved

Diagnostic testing will continue to play a key role in the eradication of obesity and is central to determining the effectiveness of treatment strategies. The proposed device will perform an immunoassay capable of detecting biomarkers for obesity, e.g., CRP, IL-6 fibrinogen and TNF-alpha. It will use a novel droplet based microfluidic system and the immunochemistry will take place on the surface of microspheres held in solution. Surface tension forces will be used to manipulate nanolitre sized droplets by applying asymmetric electric fields. This technique can successfully control biological fluids such as whole blood and urine and eliminate complex components like channels or pumps. This straightforward geometry reduces fabrication costs and makes it easier to incorporate multiple reagents. By having the immunochemistry occur on particles in solution, the device can be easily adapted to detect biomarkers for various diseases by changing the reagents. This will ensure excellent selectivity and sensitivity with fast feedback. The long term goal of this proposal is to fully quantify protein suspension behaviour in electrosprays and digital microfluidic devices towards the goal of a portable, point-of-care diagnostic test for obesity treatment monitoring using a miniaturized, microfluidic-based DMF on dielectric device coupled with high-resolution IMS.

诊断测试将继续在消除肥胖方面发挥关键作用，并对确定治疗策略的有效性至关重要。所提出的装置将执行能够检测肥胖的生物标志物的免疫测定，CRP、IL-6纤维蛋白原和TNF-α。它将使用一种新的基于液滴的微流体系统，免疫化学将在溶液中的微球表面发生。将使用表面张力通过施加不对称电场来操纵纳升大小的液滴。这种技术可以成功地控制生物流体，如全血和尿液，并消除复杂的组件，如通道或泵。这种简单的几何形状降低了制造成本，并使其更容易纳入多种试剂。通过使免疫化学发生在溶液中的颗粒上，该装置可以通过改变试剂而容易地适于检测各种疾病的生物标志物。这将确保出色的选择性和灵敏度与快速反馈。 该提案的长期目标是完全量化电喷雾和数字微流体装置中的蛋白质悬浮行为，以实现使用与高分辨率IMS耦合的电介质装置上的小型化的基于微流体的DMF进行肥胖治疗监测的便携式即时诊断测试的目标。