Characterization of probiotic bacterial cells enzyme activity, investigation of their gastrointestinal stability and impact on microbiota composition and function.

益生菌细胞酶活性的表征，研究其胃肠道稳定性以及对微生物群组成和功能的影响。

• 批准号: 523227-2018
• 负责人: Prakash, Satya
• 金额: $ 0.91万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Engage Plus Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=662563
• 关键词: Characterization; probiotic; bacterial; cells; enzyme

Probiotics are living, active microorganisms that, when ingested in certain quantities, confer significant health**benefits. Biena is a company specialized in dairy cultures, enzymes and probiotics. Biena has isolated several**natural probiotic bacterial strains and would like to develop various probiotic health formulations based on**bacterial cells enzymatic activities. Specifically Biena is looking to isolate and characterize bacterial cells for**human health benefits based on Ferulic Acid Esterase active bacterial strains. Biena lacks infrastructure and**expertise to characterize these bacterial cells for their enzymatic activities and investigate their stability in GI**transit and impact of those bacterial cells on gut bacterial population for which they approached Dr. Prakash's**lab at McGill University which has NSERC funded computer-controlled simulated human dynamic**gastrointestinal model, the only model of its kind in Canada and expertise in the field. This NSERC Engage**Plus Grant application is an extension of the existing NSERC Engage Grant between Beina and McGill**University. In this proposal characterization of probiotic bacterial cells enzyme activity, gastrointestinal**stability and impact on microbiota composition and function will be carried out. These investigations will**provide development of health food probiotic products and opportunities for collaborations.

益生菌是活的，活跃的微生物，当摄入一定数量时，会带来显着的健康益处。Biena是一家专门从事乳制品培养，酶和益生菌的公司。Biena已经分离出几种 ** 天然益生菌菌株，并希望开发基于 ** 细菌细胞酶活性的各种益生菌健康配方。具体来说，Biena正在寻求分离和表征基于阿魏酸酯酶活性菌株的细菌细胞，以 ** 人类健康益处。Biena缺乏基础设施和专业知识来表征这些细菌细胞的酶活性，并研究它们在GI** 运输中的稳定性以及这些细菌细胞对肠道细菌种群的影响，为此他们联系了麦吉尔大学的Prakash博士的实验室，该实验室拥有NSERC资助的计算机控制的模拟人体动态胃肠模型，这是加拿大唯一的同类模型和该领域的专业知识。此NSERC Engage**Plus Grant申请是Beina和麦吉尔 **University之间现有NSERC Engage Grant的扩展。在该提案中，将进行益生菌细胞酶活性、胃肠道 ** 稳定性以及对微生物群组成和功能的影响的表征。这些研究将为健康食品益生菌产品的开发和合作提供机会。