Characterization of probiotic bacterial cells enzyme activity, investigation of their gastrointestinal stability and impact on microbiota composition and function.

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Probiotics are living, active microorganisms that, when ingested in certain quantities, confer significant health**benefits. Biena is a company specialized in dairy cultures, enzymes and probiotics. Biena has isolated several**natural probiotic bacterial strains and would like to develop various probiotic health formulations based on**bacterial cells enzymatic activities. Specifically Biena is looking to isolate and characterize bacterial cells for**human health benefits based on Ferulic Acid Esterase active bacterial strains. Biena lacks infrastructure and**expertise to characterize these bacterial cells for their enzymatic activities and investigate their stability in GI**transit and impact of those bacterial cells on gut bacterial population for which they approached Dr. Prakash's**lab at McGill University which has NSERC funded computer-controlled simulated human dynamic**gastrointestinal model, the only model of its kind in Canada and expertise in the field. This NSERC Engage**Plus Grant application is an extension of the existing NSERC Engage Grant between Beina and McGill**University. In this proposal characterization of probiotic bacterial cells enzyme activity, gastrointestinal**stability and impact on microbiota composition and function will be carried out. These investigations will**provide development of health food probiotic products and opportunities for collaborations.