Assessing genetic diversity and differentiation in endangered Mottled Duskywing (Erynnis martialis) populations to support species recovery efforts

评估濒临灭绝的斑驳暗翅鸟 (Erynnis mudalis) 种群的遗传多样性和分化，以支持物种恢复工作

• 批准号: 542822-2019
• 负责人: Keyghobadi, Nusha
• 金额: $ 1.8万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=680101
• 关键词: Assessing; genetic; diversity; differentiation; endangered

The Mottled Duskywing (Erynnis martialis) is an endangered butterfly species in Canada. To reverse the contraction in its geographic range observed over the last several decades, recovery-planning documents suggest re-establishing the species at historical sites where suitable habitat remains and/or assisting colonization at previously unoccupied sites with suitable habitat. In 2015, Natural Resource Solutions Inc. (NRSI) began coordinating a multi-year collaborative project to work towards this goal by addressing key actions and knowledge gaps identified in the Draft Recovery Strategy for Mottled Duskywing. The proposed research will contribute to this multi-year collaborative recovery initiative by addressing key knowledge gaps relating to the genetics of the Mottled Duskywing. We will develop microsatellite genetic markers for Mottled Duskywing and complete genetic sampling throughout the species' range in Ontario and nearby locations in the US. This will provide information about the genetic diversity and differentiation of the Ontario subpopulations, effective population size (related to census population size), and, in conjunction with mark-recapture data being collected by collaborators, indirect estimates of gene flow. Given that genetic techniques are now widely used in conservation biology to identify source populations for reintroductions and to assess extinction risk (Frankham et al. 2002), the proposed research will directly inform Mottled Duskywing population augmentation and re-introduction efforts in Ontario.

斑驳暗翅蝶（Erynnis武术）是加拿大的一种濒临灭绝的蝴蝶。 为了扭转过去几十年观察到的地理范围的缩小，恢复规划文件建议在仍然有合适栖息地的历史遗址重建该物种和/或在具有合适栖息地的先前无人居住的地点协助殖民。 2015 年，自然资源解决方案公司 (NRSI) 开始协调一个多年期合作项目，通过解决斑色暗翼恢复战略草案中确定的关键行动和知识差距来实现这一目标。 拟议的研究将通过解决与斑驳暗翼遗传学相关的关键知识差距，为这项多年合作恢复计划做出贡献。 我们将为斑驳暗翼开发微卫星遗传标记，并在安大略省和美国附近地区的整个物种范围内完成遗传采样。 这将提供有关安大略亚种群的遗传多样性和分化、有效种群规模（与人口普查人口规模相关）的信息，并结合合作者收集的标记重新捕获数据，对基因流进行间接估计。 鉴于遗传技术现已广泛应用于保护生物学中，以识别重新引入的来源种群并评估灭绝风险（Frankham 等人，2002 年），拟议的研究将直接为安大略省的斑斑暗翅鸟种群增加和重新引入工作提供信息。