Development of a Safe and Effective COVID-19 Vaccine and Booster Using Linear Covalently Closed DNA Minivectors

使用线性共价闭合 DNA 微型载体开发安全有效的 COVID-19 疫苗和加强疫苗

• 批准号: 550127-2020
• 负责人: Sheikhzadeh, Mehdi
• 金额: $ 5.46万
• 依托单位: Lambton College of Applied Arts and Technology
• 依托单位国家: 加拿大
• 项目类别: Applied Research Rapid Response to COVID-19
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=695435
• 关键词: Development; Safe; Effective; COVID; 19

The COVID-19 pandemic is unlike anything the world has seen in over a century, both in terms of global spread and economic impact. In addition to non pharmaceutical interventions, there is an urgent need for vaccines and therapies to effectively eradicate COVID-19. Mediphage Bioceuticals located in Kitchener, ON is a genetic medicine company developing therapeutics for inherited ocular diseases, with the potential to expand to broader therapeutic areas. Their flagship technology, ministring DNA (msDNA), is a safe and effective gene delivery vector that can be used to cure genetic diseases that have limited, or no treatment options available. The objective of this proposed project in collaboration with Lambton College is to produce a safe and effective vaccine and booster using MBI's linear covalently closed (LCC) DNA minivectors. MBI and Lambton College's previous collaborations have resulted in the successful optimization of the fermentation and small scale purification processes developed by MBI for the production of LCC msDNA. To date, separation of LCC DNA from other DNA species, both by size and topology has yet to be achieved beyond analytical levels of purification. An efficient and scaled-up approach to generate large scale msDNA production is required for generation of a commercially viable vaccine. Successful completion of this project will allow Mediphage to produce the volume of msDNA-VLP necessary to conduct preclinical efficacy testing of in vitro and in vivo models. The success of this study will have colossal impacts for the future of Canadians as a vaccine is necessary to end the COVID-19 global pandemic and allow the Canadian and global economy to begin recovering.

COVID-19大流行在全球传播和经济影响方面都不同于一个多世纪以来的任何一次大流行。除了非药物干预措施外，迫切需要有效根除COVID-19的疫苗和疗法。Mediphage Bioceuticals位于安大略省基奇纳（Kitchener），是一家开发遗传性眼病治疗方法的基因药物公司，具有扩展到更广泛治疗领域的潜力。他们的旗舰技术“DNA服务”（msDNA）是一种安全有效的基因传递载体，可用于治疗治疗方案有限或没有治疗方案的遗传性疾病。