Optimization of Fermentation and Downstream Process for the Production of Lactobacillus equi Probiotics

马乳杆菌益生菌生产发酵及下游工艺的优化

• 批准号: 566179-2021
• 负责人: Sheikhzadeh, Mehdi
• 金额: $ 1.82万
• 依托单位: Lambton College of Applied Arts and Technology
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 1
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=724205
• 关键词: Optimization; Fermentation; Downstream; Process; Production

The world's only source of true ancestral probiotic strains, CanBiocin is widely recognized as a leader inspecies-specific animal and livestock probiotic development. Based in Edmonton, CanBiocin maximizes thebeneficial impact of species-specific probiotics by careful combination with prebiotic ingredients to focus onpost-biotic metabolite production to support animal health and wellness.CanBiocin wants the best health for pets and livestock that is backed by science, however, one size does not fitall when it comes to commercial fermentation and scale up as individual species-specific probiotics are diverseand have their own unique characteristics. Horses are classified as a companion animal in CanBiocin's contextand a species-specific probiotic is of immediate interest for commercial customers. To be commercially viable,the Ligilactobacillus equi (L.equi) needs further optimization of the fermentation and downstream process toimprove the product yield and purity. In collaboration, CanBiocin and Lambton College will focus ondetermining best practices that can translate into commercial-scale production and harvest of L.equi as aspecies-specific probiotic for use in supporting animal health and wellness in companion as well asperformance horses.The success of this collaborative project will allow CanBiocin to speed up their commercialization of this newproduct as there is market demand with commercial customers confirmed and awaiting delivery of thisspecialized ingredient to support microbiome health. It is anticipated that CanBiocin's operation will expand toaccommodate the additional product blending and packaging, thus leading to an additional 4 full-timepositions. Furthermore, these positions will be retained during product launch to support further productdevelopment and new species-specific strain identification and classification.

CanBiocin是世界上唯一真正的祖先益生菌菌株的来源，被广泛认为是非物种特异性动物和牲畜益生菌开发的领导者。CanBiocin总部位于埃德蒙顿，通过精心结合益生元成分，将物种特异性益生菌的有益影响最大化，专注于生物后代谢物的生产，以支持动物健康和健康。CanBiocin希望为宠物和牲畜提供最好的健康，这是有科学支持的，然而，当涉及到商业发酵和扩大规模时，一个规模并不适用，因为单个物种特定的益生菌是多种多样的，并且有自己独特的特征。在CanBiocin的背景下，马被归类为伴侣动物，一种物种特异性益生菌对商业客户具有直接的兴趣。为了使其具有商业可行性，还需要进一步优化发酵和下游工艺，以提高产品的产量和纯度。在合作中，CanBiocin和兰姆顿学院将专注于确定最佳实践，这些实践可以转化为商业规模的生产和收获，作为特定物种的益生菌，用于支持伴侣马和表演马的动物健康和保健。这一合作项目的成功将使CanBiocin加速其新产品的商业化，因为市场需求已得到商业客户的确认，并等待交付这种支持微生物群健康的专业成分。预计CanBiocin的运营将扩大，以适应额外的产品混合和包装，从而导致额外的4个全职职位。此外，这些职位将在产品发布期间保留，以支持进一步的产品开发和新的物种特异性菌株鉴定和分类。