Using Wastewater Surveillance for Detection of COVID-19 Virus

利用废水监测检测 COVID-19 病毒

• 批准号: 554592-2020
• 负责人: McBean, Edward
• 金额: $ 3.64万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=705669
• 关键词: Using; Wastewater; Surveillance; Detection; COVID

Since COVID-19 is shed in the feces of infected people, the opportunity to use surveillance of wastewater to understand the extent of COVID-19 virus within cities is enormous. This research will develop the necessary and critical protocols needed to allow a community to gain important insights of evidence-based knowledge regarding carriers of the COVID-19 virus. For example, wastewater surveillance provides the opportunity to understand the extent of COVID-19 carriers within a community (e.g. increases in COVID-19 concentrations evident in the wastewater may be translatable into understanding whether a new wave of COVID-19 is beginning, or, if concentrations of COVID-19 cases are increasing in a localized area of their municipality) Reliable procedures for wastewater surveillance are key to really understanding whether control of the virus is occurring. In addition to PCR-based detection of SARS-CoV-2, this research will also employ a metagenomic whole genome sequencing approach to provide useful data for COVID-19 surveillance. RNA extractions from concentrated sewage samples will be conducted via a series of procedures and technologies. The wastewater-based epidemiology strategy involves first transcribing coronavirus RNA into complementary DNA (cDNA) by the reverse transcriptase enzyme, then amplifying the resultant DNA via real time polymerase chain reaction (RT-PCR) to improve signal detection. Sequencing techniques will be needed to confirm viral presence in wastewater samples in sufficiently large numbers to be indicators. Ultimately, the intent is to provide governments at all levels, with reliable procedures for wastewater surveillance to really understand whether control of the virus is occurring.

由于COVID-19是在感染者的粪便中传播的，因此利用废水监测来了解城市内COVID-19病毒的范围的机会是巨大的。这项研究将制定必要和关键的方案，使社区能够获得关于COVID-19病毒携带者的循证知识的重要见解。例如，废水监测为了解社区内COVID-19携带者的程度提供了机会(例如，废水中COVID-19浓度的明显增加可以转化为了解新一波COVID-19是否正在开始，或者，如果其所在城市的局部地区COVID-19病例浓度增加)，可靠的废水监测程序是真正了解病毒是否得到控制的关键。