Identification of bioactive peptides from digestion of collagen hydrolysates

从胶原蛋白水解物的消化中鉴定生物活性肽

• 批准号: 535744-2018
• 负责人: Kubow, Stan
• 金额: $ 4.37万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=720038
• 关键词: Identification; bioactive; peptides; digestion; collagen

Genacol Canada Corporation Inc. manufactures a bovine-sourced hydrolyzed collagen supplement (Genacol®Original Formula), which has shown efficacy in two separate clinical trials for reducing joint pain in subjects with osteoarthritis (OA). Following digestion, hydrolyzed collagen yields amino acids and peptides that can lead to the health promoting properties of the supplement. There is presently no information, however, regarding the peptide and amino acid profiles generated from the digestion of the Genacol supplement. Key information is also lacking regarding the collagen peptides reaching the joint and their mechanisms of action. In the present proposal, the profiles of the peptides and amino acids generated from digestive enzyme-mediated hydrolysis of the Genacol collagen product will be investigated using the dynamic simulated gut digestion model. To identify bioavailable peptides, the peptide fractions of the CH digests obtained from a dynamic simulated human GI model will be exposed to a human intestinal and liver cell co-culture system that simulates peptides that would reach the blood circulation. The peptides obtained from the co-culture will be studied for their anti-inflammatory effects in human OA articular cartilage tissue. Also, peptides generated during the simulated digestion studies will be investigated for their anti-inflammatory effects involving a co-culture of human intestinal cells, immune cells and intestinal dendritic cells involved in controlling pro-inflammatory factors that could also compromise joint health. This approach will help to ascertain the bioactive components contributing towards the health promoting properties of the Genacol collagen supplement and will aid in the development of more concentrated and potent hydrolyzed collagen products. Based on the profiles of bioavailable peptides shown to be bioactive in the OA cartilage tissue explants, Genacol will manufacture CH formulations enriched in those peptides that will be tested for their bioavailability and bioactivity in the above testing systems.

Genacol Canada Corporation Inc.生产了牛源水解的胶原蛋白补充剂（Genacol®OriginalFormula），该补充剂在两项单独的临床试验中显示出效率，用于减轻骨关节炎（OA）受试者的关节疼痛。消化后，水解胶原蛋白会产生氨基酸和胡椒粉，从而导致补充剂的健康促进特性。但是，目前尚无有关因基因加尔补充剂消化而产生的肽和氨基酸谱的信息。关于胶原蛋白肽达到关节及其作用机理的关键信息也缺乏。在本提案中，将使用动态模拟的肠道消化模型研究了由消化酶介导的水解产生的肽和氨基酸的剖面。为了鉴定可生物利用的辣椒，从动态模拟的人类GI模型中获得的CH消化的胡椒部分将暴露于人类肠道和肝细胞共培养系统，该系统模拟将达到血液循环的肽。将研究从共培养物获得的辣椒，以研究其在人OA关节软骨组织中的抗炎作用。同样，将研究在模拟消化研究期间产生的肽，以涉及涉及人类肠细胞，免疫细胞和肠道树突状细胞共同培养参与控制促炎性因素的抗炎作用，这也可能损害关节健康。这种方法将有助于确定促进Genacol胶原蛋白补充剂健康特性的生物活性成分，并有助于开发更浓缩和潜在的水解胶原蛋白产品。基于在OA软骨组织外植体中证明具有生物活性的生物可用辣椒的曲线，Genacol将生产富含那些辣椒的CH配方，这些配方将在上述测试系统中对其生物利用度和生物活性进行测试。