Engineering Research Equipment Grant: Liquid Scintillation Counter

这项研究的目的是了解微生物和哺乳动物细胞的基本代谢，并利用这些信息来改变反应系统的生产力。液体闪烁计数器将用于以下项目：(1)生物聚合物颗粒对细菌生物膜代谢和生态的影响；(2)底物组成和生长速率的变化可提高多形羊草的酶产率；(3)黏附对地下水中外源化合物降解基因工程微生物代谢的影响；(4)次生代谢产物质量、产量和生产速率的热化学控制——油菜黄原菌合成多糖黄原胶；(5)杂交瘤产生单克隆抗体的动力学及优化工程研究。

This research effort is aimed at understanding the basic metabolism of microbial and mammalian cells and to use that information to alter the productivity of the reaction system. A liquid scintillation counter will be used for the following projects: (1) effects of biopolymer particulates on the metabolism and ecology of bacterial biofilms; (2) enhanced enzyme productivity in Hansenula polymorpha by substrate composition and growth rate transients; (3) influence of adhesion on the metabolism of microbes genetically-engineered for xenobiotic compound degradation in the ground water; (4) thermochemical control of secondary metabolite quality, yield, and production rate-synthesis of the polysaccharide Xanthan by Xanthamonas campestris; and (5) engineering studies of kinetics and optimization of monoclonal antibody production by a hybridoma.