Development of a Rational Basis for Scaling Bioreactors for Production of Proteins from Cloned Eukaryotic Genes and Viral Pesticides Using Insect Cell Cultures

昆虫细胞培养提供了比哺乳动物细胞培养更大的潜在优势 用于从重组DNA生产蛋白质的细胞培养物。 它们还提供了优于使用幼虫的优点， 生产病毒杀虫剂。 高效的大规模工艺 需要制定利用这些潜力的战略。 两 关键的障碍是对细胞如何 生长和病毒繁殖机制响应于 文化条件，以及无法翻译这些知识 设计和操作易于扩展的生物反应器。 一个 建议进行综合研究，以减少这些障碍。 还将开发流体动力学和动力学模型， 验证

Insect cell cultures offer potential advantages over mammalian cell cultures for production of proteins from recombinant DNA. They also offer advantages over the use of larvae for the production of viral insecticides. Efficient large scale process strategies to exploit these potentials need to be developed. Two key barriers are an inadequate understanding of how cellular growth and viral reproductive mechanisms respond to changes in culture conditions, and an inability to translate such knowledge into the design and operation of easily scalable bioreactors. An integrated research effort is proposed to reduce these barriers. Fluid dynamic and kinetic models are also to be developed and verified.