Engineering Research Equipment Grant: Attenuated Total Reflectance/Fourier Transform Infrared Spectrometer Purchase

一台具有衰减全反射(ATR)功能的傅立叶变换红外(FT-IR)光谱仪将被购买，供生化工程中心的教职员工专用。工程学院内部不存在这样的工具。最近的FT-IR光谱仪位于杜克大学化学系，专门用于非生物样品。目前需要ATR/FT-IR光谱仪的主要研究人员正在进行的和未来的项目包括：细菌黏附和生物膜形成的原位ATR/FT-IR光谱1.细菌黏附与固定化细胞生理学的分子化学2.细菌生物膜中的大分子传质3.细菌生物膜脱落的机理蛋白质吸附膜的ATR/FT-IR光谱研究1.蛋白质在吸附过程中的变性2.基质的物理化学性质对蛋白质吸附膜的影响3.依赖锚定的哺乳动物细胞突起的ATR/FT-IR光谱研究1.底物预处理对锚定依赖性哺乳动物细胞黏附的影响2.剪切力和生长因子对锚定依赖性哺乳动物细胞生理的影响

A FOURIER TRANSFORM INFRARED (FT-IR) Spectrometer with Attenuated Total Reflectance (ATR) capabilities is being purchased for the dedicated use of faculty within the Center for Biochemical Engineering. No such instrument exists within the School of Engineering. The nearest FT-IR spectrometer resides in the Chemistry Department at Duke and its use is dedicated to non-biological samples. On-going and future projects of the principal investigators currently requiring an ATR/FT-IR spectrometer include: In-Situ ATR/FT-IR SPECTROSCOPY OF BACTERIAL ADHESION AND BIOFILM FORMATION 1. Molecular Chemistry of Bacterial Adhesion and Immobilized Cell Physiology 2. Macromolecule Mass Transport Through Bacterial Biofilms 3. Mechanisms Governing Bacterial Biofilm Sloughing ATR/FT-IR SPECTROSCOPY OF ADSORBED PROTEIN FILMS 1. Protein Denaturation Upon Adsorption 2. Effects of Physiochemical Nature of Substratum on Adsorbed Protein Films 3. Mechanisms of Ligand binding to Adsorbed Proteins ATR/FT-IR SPECTROSCOPY OF ANCHORAGE-DEPENDENT MAMMALIAN CELL PROCESSES 1. Substratum Pretreatment Effects on Anchorage-Dependent Mammalian Cell Adhesion 2. Shear Stress and Growth Factor Effects on Anchorage- Dependent Mammalian Cell Physiology