Genetic Analysis and Metabolic Engineering of Anaerobic Butyric-Acid Clostridia

这项研究的目的是为了产生新的 分子遗传学和酶学知识， 厌氧丁酸梭菌 细胞代谢的有益遗传改变 （代谢工程）用于各种应用。 丁酸梭菌具有很好的潜力， 用于生产日用品和特种产品的工业应用 化学品 目前的知识基础上的分子 遗传学、细胞调节和代谢工程 梭状芽孢杆菌细胞非常狭窄，但结果是扩张 主要研究人员目前的努力， 提议 拟议的研究包括建造一个 各种酶途径改变的细胞， 分批、连续和连续地测试这些电池， 细胞再循环生物反应器，以研究这些 细胞和重组酶在 产物形成

The objectives of the proposed research are to produce new knowledge on the molecular genetics and enzymology of anaerobic butryic-acid clostridia that will allow beneficial genetic alterations of cell metabolism (metabolic engineering) for a variety of applications. Butryic-acid clostridia have an excellent potential for industrial applications to produce commodity and specialty chemicals. The current knowledge base on the molecular genetics, cell regulation and metabolic engineering of clostridia cells is very narrow, but expanding as a result of current efforts by the Principal Investigators of this proposal. Proposed studies include construction of a variety of cells with altered enzymatic pathways and testing of these cells in batch, continuous and cell-recycle bioreactors to study the stability of these cells and the effectiveness of the recombinant enzymes in product formation.