High Voltage Electrophoresis in Capillary Tubes

这项研究属于分析和表面化学的一般领域，也属于分离科学的子领域。本研究致力于毛细管区带电泳法在生物样品复杂混合物分析中的应用研究。将对几个主要研究领域进行研究，包括1)在数十秒的时间框架内进行快速分离，2)结合液相色谱和毛细管区带电泳(CZE)进行二维分离，3)通过自动CZE系统获取物理化学数据/迁移预测，以及4)超微尺度分析。这项研究利用高压电场(电泳法)中电荷物种的迁移，结合毛细管柱的新发展来实现带电物种的有效分离。这项新技术将进一步发展并应用于复杂生物样品的分析。

This research is in the general area of analytical and surface chemistry and in the subfield of separation science. This research focuses on the continued development of capillary zone electrophoresis for analysis of complex mixtures from biological samples. Several major areas of research will be investigated including 1) fast separations in a time frame of tens of seconds, 2) two-dimensional separations involving a combination of liquid chromatography and capillary zone electrophoresis (CZE), 3) acquisition of physicochemical data / migration prediction via an automated CZE system, and 4) ultra- microscale analysis. This research uses charge species migration in high voltage fields (electrophoresis) in combination with new developments in capillary columns to achieve efficient separation of charged species. This new technology will be further developed and applied to the analysis of complex biological samples.