Molecular Approaches to the Phylogeny of Leguminosae

豆科植物是开花植物的第三大科，包括许多生态和经济上重要的物种。为了理解这种巨大的多样性，有必要描述将许多物种和属彼此联系起来的关系和系统发育模式。这种类型的许多工作已经完成，并继续使用传统的字符，如形态学和化学。本研究将集中在DNA水平上的变异模式，特别强调叶绿体基因组，这已被证明是构建系统发育假设的重要特征来源。限制性位点分析和DNA测序将用于调查代表关键亚科Papilionoideae和Caesalpinioideae所有主要部落的属，这些亚科被认为代表了该家族的主要辐射。这些研究的结果将与社区其他地方正在进行的非分子研究结果相结合。由此产生的系统发育假说将用于研究家族中固氮共生的起源，重点是涉及结瘤的核多基因家族的发展。

Leguminosae is the third largest family of flowering plants, and includes many ecologically and economically important species. To understand this great divesity it is necessary to describe the patterns of relationship and phylogeny linking the many species and genera to one another. Much work of this type has been done, and continues, using traditional characters such as morphology and chemistry. This study will focus on variation patterns at the DNA level, specifically emphasizing the chloroplast genome, which has proven to be of great utility as a source of characters for constructing phylogenetic hypotheses. Restriction site analysis and DNA sequencing will be used to survey genera representing all of the major tribes of the pivotal subfamilies Papilionoideae and Caesalpinioideae, the groups considered to represent the main radiation of the family. Results from these studies will be integrated with those of non-molecular studies progressing elsewhere in the community. The resulting phylogenetic hypotheses will be used to study the origins of nitrogen-fixing symbioses in the family, with emphasis on the development of the nuclear multigene families involved in nodulation.