Acquisition of a Confocal Laser Scanning Microscope

9202417摩尔：此提案要求为多用户组使用的共聚焦显微镜申请资金。主要用户将长期在项目中使用该仪器。项目包括分化过程中变化的染色体结构域和特定的和新发现的核结构域的三维重建，通过维甲酸或皮肤重建共培养在发育或诱导分化过程中细胞之间变化的相互关系，整个发育胚胎中mRNA的不同分布，以及完整细胞核中特定基因的相对定位（制图）。除了这些较大的项目外，Wistar研究所的不同实验室还确定并启动了一些小项目，特别是正在发展的结构生物学项目。这些将由首席研究员监督。项目是多种多样的，需要一种有效的方法来研究厚度为0.1-1毫米的标本在0.1至1米范围内的细胞或组织结构。共聚焦显微镜的先进状态为我们提供了实现目标的手段。

9202417 Maul This proposal requests the funds for a confocal microscope to be used by a multi-user group. The major users will utilize the instrument in projects over the long term. Projects include the 3D reconstruction of changing chromosomal domains during differentiation and of specific and newly found nuclear domains, the changing inter- relationships of cells during development or induced differentiation by retinoic acids or by coculture in skin reconstruction, the different distribution of mRNA in whole developing embryos, and the relative localization of specific genes in the intact nucleus (mapping). Aside from these larger projects minor projects have been identified and initiated by different laboratories at The Wistar Institute in particular in the developing structural biology program. These will be supervised by the principal investigator. Projects are diverse and need an efficient way to study cell or tissue architecture in the 0.1 to 1 m range in specimens that have a thickness of 0.1-1-mm. Confocal microscopy in its advanced state provides us with the means to achieve our goals.