Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia

该项目的目标是产生厌氧丁酸梭菌的分子遗传学和酶学方面的新知识，这将允许对各种细菌的细胞代谢进行有益的遗传改变应用程序。丁酸梭菌在生产商品和特种化学品方面具有良好的工业应用潜力。目前关于这些细胞的遗传、调节和代谢的知识基础是有限的，但是在以前的资助下已经取得了相当大的进展。本项目将继续开展梭状芽孢杆菌基因调控的研究，进一步发展梭状芽孢杆菌的表达系统，并评估参与梭状芽孢杆菌代谢途径调控的其他因素。

The objectives of this project are to produce new knowledge on the molecular genetics and enzymology of anaerobic butryic clostridia that will allow beneficial genetic alterations of the cell metabolism for a variety of applications. Butryic acid clostridia have an excellent potential for industrial applications to produce commodity and specialty chemicals. The cur- rent knowledge base on the genetic- s, regulation and metabolism of these cells has been limited, but considerable progress has been made by work on previous grants. This project will continue the study of gene regulation, develop further the expression systems in clostrid- ia, and evaluate other factors in- volved in the regulation of metabo- lic pathways in clostridia.