Acquisition of a Confocal Scanning Imaging System

9220230 Kempf奥本大学提议以50%的费用分摊购买一台激光扫描共聚焦显微镜。该显微镜将用于以下研究：卵黄形成和内吞作用、神经发育、受体-配体结合、神经系统分化过程中激酶和底物的区隔化作用、Na/K atp酶和碳无水在水平衡中的生理功能、神经网络建模、超高速微流星撞击、寄生虫组织囊肿形成、高温辐射技术。以及荷尔蒙对生殖器官分化的控制。这项研究将使用多种技术，包括三维重建，地形成像，通过使用重要的荧光染料分析代谢功能，以及分析钙的分布和通量。该显微镜将满足奥本大学许多教师的需求，他们的研究项目需要该仪器的独特功能。其中包括一些研究人员，他们的研究工作受到阻碍，因为他们必须长途跋涉到雅典的佐治亚大学才能获得这种显微镜的使用。提议的仪器将被安置在奥本大学细胞科学中心，在那里它将随时可供提交该提案的pi以及其他教师和学生使用。需要共聚焦显微镜的研究人员来自许多不同的部门，代表不同的学科，包括原生动物学、发育生物学、细胞生物学、生理学、分子生物学、寄生虫学和电子工程

9220230 Kempf The purchase of a laser, scanning, confocal microscope is proposed with a 50% cost-share by Auburn University. The microscope will be utilized for research concerned with vitellogenesis and endocytosis, neurodevelopment, receptor-ligand binding, the role of compartmentalization of kinase and substrate during nervous system differentiation, physiological function of Na/K ATPase and carbonic anhydrace in water balance, neural network modeling, hypervelocity micrometeoroid impacts, tissue cyst formation by parasites, high temperature radiator technology, and the hormonal control of reproductive organ differentiation. This research will use a variety of techniques including 3-dimensional reconstruction, topographic imaging, analysis of metabolic function via the use of vital fluorescent dyes, and analysis of calcium distribution and flux. The microscope will serve the needs of many faculty at Auburn University whose research programs require the unique capabilities of this instrument. This includes a number of investigators whose research efforts are hampered by having to travel a considerable distance to University of Georgia, Athens to gain the use of such a microscope. The proposed instrumentation will be housed in the Auburn University Cell Science Center where it will be readily available for use by the PIs submitting this proposal, as well as by other faculty and students. Investigators that have need of a confocal microscope are from a number of different departments and represent diverse disciplines including Protozoology, Developmental Biology, Cell Biology, Physiology, Molecular Biology, Parasitology, and Electrical Engineering