Acquisition of Instrumentation for Genomics Research

该提案旨在获得在普渡大学校园进行基因组学研究所需的仪器。普渡大学在过去7年中开展了几项重要的倡议，为遗传学研究提供了大量的力量。这些包括建设和改造重要的实验室空间，建立一个跨学科的遗传学研究生培训计划，以及雇用几位具有广泛和令人兴奋的主题的专业知识的遗传学教师。这种不断增长的势头现在使普渡大学合适地建立了普渡基因组学实验室（PGL）。这样一个实验室一旦具备高通量基因组数据生成和分析能力，将服务于三个重要目标：支持8个主要用户的基因组学研究工作；作为学生培训设施，支持nsf资助的植物遗传学研究生培训工作；促进基于dna的方法在植物改良中的应用的合作联系。满足这些目标所需的仪器包括电子放射自显影系统、带有分子生物学实验室的自动DNA测序系统、凝胶记录系统、CHEF制图系统、热循环仪、-80℃冷冻设备和植物生长室设施。这种设备一旦到位，就可以启动几个大规模的基因组学研究项目，研究重要的植物和真菌基因组，这些基因组迄今为止还没有得到很好的表征。目前，普渡大学校园缺乏这些设施，阻碍了这些有价值的研究努力。

This proposal seeks to acquire instrumentation needed to facilitate genomics research on the Purdue University campus. Purdue University has undertaken several important initiatives over the past 7 years that have provided substantial strength to genetics research. These include the construction and renovation of significant laboratory space, establishment of an interdisciplinary graduate training program in genetics, and the hiring of several genetics faculty with expertise spanning a broad and exciting range of topics. This growing momentum has now appropriately positioned Purdue University for the establishment of the Purdue Genomics Laboratory (PGL). Such a laboratory, once equipped for high throughput genomics data generation and analysis, will serve three important objectives: To support genomics research efforts of 8 primary users, to serve as a student training facility in support of a NSF-sponsored graduate training effort in plant genetics, and to facilitate collaborative linkages for the application of DNA-based methodologies to plant improvement. The instrumentation needed to meet these objectives includes an electronic autoradiography system, an automated DNA sequencing system with molecular biology labstation, a gel documentation system, CHEF mapping systems, thermocyclers, -80C freezer equipment, and plant growth chamber facilities. This equipment, once in place, would allow the initiation of several large-scale genomics research projects in important plant and fimgal genomes that, to date, have not been well-characterized. At present, the lack of these facilities on the Purdue campus are all that stand in the way of these valuable research endeavors.