High Voltage Electrophoresis in Capillary Tubes

该项目由分析和表面化学项目支持，旨在开发新的仪器和方法，以提高毛细管电泳（CE）的能力。一种新的双光束吸收几何结构被采用，其中两个光电二极管阵列被用来获得空间分辨检测，同时校正背景吸收和源漂移。基于流体静力学驱动的液体流动，流量平衡CE的发展旨在提高可探测性和分辨率。射流界面是一种快速的样品导入方法。尺寸排除色谱被用作一种额外的分离手段之前，目前采用液相色谱- ce多维方法学。最后，研究了超高压（300千伏）的发展方法，试图开辟一种新的CE运行模式。分离复杂样品成分的方法是生物和环境分析发展中不可或缺的一部分。乔根森教授的实验室在开发毛细管电泳新仪器方面处于领先地位。毛细管电泳是一种非常重要的生物样品分析工具，如血液和尿液，在环境样品分析中也越来越多地使用。在这个领域的工作已经导致了更快、更敏感和更可靠的分析。乔根森教授和他的学生所做的工作很可能会为复杂的化学分析带来新的方法和仪器。

This project, supported by the Analytical and Surface Chemistry Program, is directed at the development of new instrumentation and methodologies to enhance the capabilities of capillary electrophoresis (CE). A novel double-beam absorbance geometry is employed whereby two photodiode arrays are used to obtain spatially resolved detection while correcting for background absorbance and source drift. Development of flow counterbalanced CE, based on a hydrostatically driven liquid flow, is directed at enhancing detectability and resolution. A jet flow interface is being investigated as a rapid means of sample introduction. Size exclusion chromatography is used as an additional separation means prior to the presently employed liquid chromatography-CE multidimensional methodology. Finally, the development of methods for using ultra-high voltages (300 kV) is investigated in an attempt to open a new mode of CE operation. Methods of separating the constituents of complex samples are integral to developments in biological and environmental analyses. Professor Jorgenson's laboratory is a leader in the development of new instrumentation for capillary electrophoresis. Capillary electrophoresis is a very important tool in the analysis of biological samples, such as blood and urine, and is finding increased usage in the analysis of environmental specimens as well. Work in this area has led to faster, more sensitive, and more reliable analyses. The work to be done by Professor Jorgenson and his students is likely to result in new approaches and instrumentation for complex chemical analyses.