Acquisition of a Confocal Laser Scanning Microscope for Cell and Neurobiologists

该提案涉及为一群细胞、分子和神经生物学家购买共聚焦激光扫描显微镜(CLSM)和图像分析系统，他们的工作重点是细胞黏附和运动、基因表达、蛋白质功能和定位以及神经发育。选择这些部件是为了提供1)用于可见光光谱中不同荧光染料的共聚焦荧光成像的能力，2)利用CLSM记录匹配的DIC图像以帮助荧光标记结构的精确定位的能力，3)在样本中的多个位置处获取延时共焦图像并自动聚焦在结构上的能力，4)使用视频-DIC作为评估延时CLSM图像的框架来记录细胞运动的能力，5)存储数字图像并将其传送到第二工作站以供进一步分析的能力，6)将图像大容量存储在计算机盘上并写入CD的能力，以及7)打印出版物质量的彩色和黑白缩微图的能力。

The proposal involves the acquisition of a confocal laser scanning microscope (CLSM) and image analysis system for a group of cell, molecular and neurobiologists whose work focuses on cell adhesion and movement, gene expression, protein function and localization and neural development. The components were chosen to provide 1) capability for confocal fluorescence imaging for different fluorochromes in the visible spectrum, 2) ability to record matching DIC images with the CLSM to assist in precise localization of fluorescent labelled structures, 3) an ability to take time-lapse confocal images at multiple positions in a specimen and autofocus on the structures, 4) an ability to record movements of cells using video-DIC as a framework against which time-lapse CLSM images can be evaluated, 5) an ability to store digital images and transfer them to a second work station for further analysis, 6) a capability for mass storage of images on computer disks and for writing to CDs, and 7) the ability to print publication quality color as well as black and white micrographs.