A Microfabricated Cell Sorter for Molecular Evolution

该SGER提案的总体目标是确定是否可行构建一个经济的、微制造的细胞分选器，用于分选“定向进化”实验中产生的大量细菌群体。将确定一种适当的弹性体材料，以最大限度地减少细菌细胞对分选机表面的粘附。将确定一种分选方法（识别和分离标记细胞），并开发相应的软件。将测定仪器中细菌细胞的生存能力。地震实验室（加州理工学院应用物理）的领先优势促进了这一通常的重大努力，该实验室具有类似的设计，可以根据单个DNA分子的荧光特性对其进行大小和分类。

The overall goal of this SGER Proposal is to determine if it is feasible to construct an economical, microfabricated cell sorter for sorting large numbers of bacterial populations generated in "directed evolotion" experiments. An appropriate elastomeric material will be identified that minimizes the adherence of bacterial cells to the sorter surface. A sorting method (identifies and separates tagged cells) will be identified and the appropriate software developed. The viability of the bacterial cells in the apparatus will be determined. This normally major effort is facilitated by the head-start the Quake Laboratory (Applied Physics, Caltech) has with a similar design for sizing and sorting single DNA molecules based on their fluorescent properties.