ME: Metabolic Engineering of Solvent Tolerance in Anaerobic Bacteria
ME: Metabolic Engineering of Solvent Tolerance in Anaerobic Bacteria
批准号:
9911231
负责人:
Eleftherios Papoutsakis
金额:
$34.42万
依托单位:
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-07-01 至 2003-08-31
中文摘要
本项目的目的是了解丙酮丁醇梭菌溶剂耐受性的分子基础,目的是利用这些信息获得产生更高浓度溶剂的菌株。 该研究围绕两个假设组织:(1)生产高浓度溶剂(丁醇)的能力,以及丁醇耐受性,通过过表达热休克蛋白的菌株中蛋白质稳定性的增加而增强;和(2)丁醇耐受性通过天然溶剂/耐药基因的表达而增强,例如。G.多药外排家族的蛋白质。 该项目由NSF的两个项目和环境保护署(EPA)共同资助。
英文摘要
The objective of this project is to understand the molecular basis of solvent tolerance in Clostridium acetobutylicum, with the purpose of using this information to obtain strains that produce higher concentrations of solvents. The research is organized around two hypotheses: (1) that the ability to produce high concentrations of solvent (butanol), and also butanol tolerance, is enhanced by increased protein stability in strains that overexpress heat-shock proteins; and (2) that butanol tolerance is enhanced by expression of native solvent/drug resistance genes, e. g. proteins of the multidrug efflux family. This project is being jointly funded by two programs at NSF and by the Environmental Protection Agency (EPA).
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批准号:1804741
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项目类别:Standard Grant
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资助金额:$50.0万
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财政年份:2018
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批准号:1033926
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批准号:0853490
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财政年份:2009
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负责人:Eleftherios Papoutsakis
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依托单位:
Collaborative Research: Genomic Approaches to Metabolic Engineering of Solventogenic Clostridia
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批准号:0824629
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项目类别:Continuing Grant
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资助金额:$12.48万
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财政年份:2008
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负责人:Eleftherios Papoutsakis
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依托单位:
Multilibrary Strategies for Developing Complex Transgenic Microbial Phenotypes
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批准号:0756451
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项目类别:Standard Grant
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资助金额:$0.0万
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财政年份:2008
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依托单位:
Collaborative Research: Genomic Approaches to Metabolic Engineering of Solventogenic Clostridia
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批准号:0418157
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项目类别:Continuing Grant
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资助金额:$79.98万
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财政年份:2004
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负责人:Eleftherios Papoutsakis
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依托单位:
BIochemical Engineering XIII (BioChE-03)
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批准号:0331063
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项目类别:Standard Grant
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资助金额:$2.0万
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财政年份:2003
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负责人:Eleftherios Papoutsakis
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依托单位:
Metabolic Engineering (ME): Genomic and Genetic Approaches to Solvent Tolerance in Anaerobes
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批准号:0331402
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项目类别:Continuing Grant
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资助金额:$60.75万
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财政年份:2003
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负责人:Eleftherios Papoutsakis
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依托单位:
Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
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批准号:9905669
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项目类别:Continuing Grant
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资助金额:$81.83万
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财政年份:2000
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负责人:Eleftherios Papoutsakis
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依托单位:
Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
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批准号:9632217
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项目类别:Continuing Grant
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资助金额:$53.11万
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财政年份:1996
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负责人:Eleftherios Papoutsakis
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依托单位:
Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
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批准号:9210108
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项目类别:Continuing Grant
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资助金额:$68.79万
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财政年份:1992
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负责人:Eleftherios Papoutsakis
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依托单位:
Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
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批准号:9209627
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项目类别:Continuing Grant
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资助金额:$55.5万
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财政年份:1992
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负责人:Eleftherios Papoutsakis
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依托单位:
Understanding Injury of Suspended Animal Cells in Mixed Bioreactors Due to Bubble Breakup
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批准号:9022472
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项目类别:Continuing Grant
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资助金额:$19.06万
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财政年份:1991
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负责人:Eleftherios Papoutsakis
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依托单位:
Genetic Analysis and Metabolic Engineering of Anaerobic Butyric-Acid Clostridia
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批准号:8912209
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项目类别:Continuing Grant
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资助金额:$43.36万
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财政年份:1989
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负责人:Eleftherios Papoutsakis
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依托单位:
Genetic Analysis and Metabolic Engineering of Anaerobic Butyric-Acid Clostridia
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批准号:8912094
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项目类别:Continuing Grant
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资助金额:$25.94万
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财政年份:1989
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负责人:Eleftherios Papoutsakis
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依托单位:
Instrumentation for Research in Animal-Cell Culture and Metabolic Engineering
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批准号:8906388
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项目类别:Standard Grant
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资助金额:$4.9万
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财政年份:1989
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负责人:Eleftherios Papoutsakis
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依托单位:
Presidential Young Investigator Award: Studies in Biochemical Engineering
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批准号:8896100
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项目类别:Continuing Grant
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资助金额:$21.9万
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财政年份:1987
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依托单位:
Recombinant-DNA Alterations, and Genetic and Enzymatic Characterization of Anaerobic Fermentation Process Importantto Biocatalysis by Butric-Acid Bacteria (Clostridia)
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批准号:8613077
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项目类别:Continuing Grant
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资助金额:$55.43万
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财政年份:1986
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负责人:Eleftherios Papoutsakis
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依托单位:
Enzymatic Mechanisms and Regulation of Solvent Production in Butanol/Acetone Fermentation
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批准号:8518959
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项目类别:Continuing Grant
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资助金额:$11.0万
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财政年份:1986
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负责人:Eleftherios Papoutsakis
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依托单位:
国内基金
海外基金
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批准号:81930042
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项目类别:重点项目
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资助金额:305.0万元
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批准年份:2019
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负责人:王迪
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依托单位: