Integration of Liquid-Chromatography Mass Spectrometry Across the Chemistry Curriculum

化学（12）结合液相色谱法（LC）和质谱法的分析方法是学术，制药和工业研究实验室研究的一个组成部分。虽然LC/MS在与仪器和定量分析相关的本科分析讲座课程中进行了讨论，但本科生很少获得LC/MS仪器的任何“实践”经验。我们正在整合到我们的本科课程三个实验室实验，改编自研究文献，利用电喷雾电离（ESI）和LC/离子阱MS作为分析组件。化学专业的学生在有机化学的第二学期学习LC/MS，在那里他们研究各种氨基酸在固相肽合成中的反应性。本科生物化学专业的学生使用ESI和LC/Ion Trap MS来测量蛋白质甘油醛-3-磷酸脱氢酶的分子量及其胰蛋白酶片段的质量。我们的仪器分析实验室的学生将ESI和离子阱MS与ICP-四极杆MS和其他分析方法进行比较，以确定当地环境中的铅。此外，本科生在他们的研究项目中使用ESI和LC/ Ion-Trap MS。

Chemistry (12) Analytical methods incorporating liquid chromatography (LC) and mass spectrometry are an integral part of research in academic, pharmaceutical, and industrial research laboratories. While LC/MS is discussed in undergraduate analytical lecture courses associated with Instrumental and Quantitative analysis, undergraduates rarely obtain any "hands-on" experience with LC/MS instrumentation. We are integrating into our undergraduate curriculum three laboratory experiments, adapted from the research literature, that utilize electrospray ionization (ESI) and LC/ Ion-Trap MS as an analytical component. Chemistry majors are introduced to LC/MS in their second semester of organic chemistry where they study the reactivity of various amino acids in solid phase peptide synthesis. Undergraduate biochemistry students use ESI and LC/Ion Trap MS to measure the molecular weight of the protein glyceraldehyde-3-phosphate dehydrogenase and the masses of its tryptic fragments. Students in our instrumental analysis laboratory compare ESI and Ion Trap MS to ICP-quadrupole MS and other analytical methods for the determination of lead in the local environment. In addition, undergraduate students are using ESI and LC/ Ion-Trap MS in their research projects.