A lab-on-nanotips composed out of an array of lab-on-molecules - A Multidimensional ECL Analytical Laboratory at Fiber Optical Multi-Tips (MULTIDIM-LAB)

这是波尔多和锡根的团队之间的这个联合项目的目标和愿景，在多纤维光纤束的6000个纳米尖端（尖端实验室）上安装一个多面本质的高密度传感器阵列，从而通过电化学发光（ECL）和光致发光（PL）实现n维（生物）化学分析。由于ECL已成为免疫测定、食品/水测试和许多单个分析物的强大定量分析技术，目前的努力旨在在具有6000根单独光纤的300 μm（直径）光波导的全球尖端建立一个完整的分析实验室。为了实现这一目标，需要在单独引导和空间高度限定的电聚合/功能化过程中处理光纤纳米尖端（直径：3 μm;顶点：30 nm曲率半径），使得每个单独的尖端可以配备有通过电聚合物主链导电连接到电活性表面的不同的传感ECL系统。开发这样一个复杂的尖端实验室系统需要光纤系统的技术进步（波尔多：在每根光纤中注入光，在导电的ITO涂层纳米尖端上进行电聚合，在纳米尺度上进行电致图案化）和全新的有机技术（Siegen：电和光响应单体，新的ECL/PL探针，它们本身构成了分子实验室）。安装的ECL/PL传感器越多，就越有可能通过双通道检测以定量方式分析甚至最复杂的分析物混合物，特别是在使用基于概率神经网络的训练方法之后。

It is the goal and vision of this joint project between groups in Bordeaux and Siegen to mount a high-density sensoric array of multifaceted essence at the 6000 nanotips of a multi-fiber optical bundle (lab-on-tips) thus enabling n-dimensional (bio)chemical analysis by electrochemiluminescence (ECL) and photoluminescence (PL). As ECL has become a powerful quantitative analytical technique for immunoassays, food / water testing and many individual analytes, the present endeavour seeks to establish a full analytical laboratory at the global tip of a 300 μm (diameter) optical waveguide with its 6000 individual fibers. To accomplish this goal, the fiber optical nanotips (diameter: 3 μm; apex: 30 nm curvature radius) need to be addressed in an individually guided and spatially highly defined electropolymerization / functionalization process, such that each individual tip may be equipped with a distinct sensoric ECL system conductively connected to the electroactive surface by an electropolymeric backbone. The development of such a sophisticated lab-on-tips system requires technological progress of the fiber optical system (Bordeaux: light injection in each single fiber, electropolymerisation at the conductive ITO-coated nanotips, photopatterning at the nanometric scale) and fundamentally new organic technologies (Siegen: electro-and photoresponsive monomers, new ECL/PL probes that themselves constitute lab-on-molecules). The more individual ECL/PL sensors are mounted, the more it should be possible to analyze even utmost complex analyte mixtures in a quantitative manner by twochannel detection, in particular after use of training methods based on probabilistic neural networks.