Kartenbasierte und Funktionsorientierte genomische Ansätze zur Analyse der Vererbung und Ausprägung von Merkmalen des Wasserbindungsvermögens in kommerziellen Schweinepopulationen

在这个项目中，我们将调查基因的基础上滴水损失的变化，在商业猪种群。一方面，我们将继续分析在DRIP研究单位的初始阶段确定的候选基因。另一方面，我们还将采用新的尖端实验策略，这些策略仅在过去两年中可用。我们计划使用平铺微阵列研究所有转录本的表达，包括QTL间隔的选定区域中的非编码RNA。通过这种方法，我们将寻找表型分歧和QTL基因型分歧猪之间的表达模式的差异。此外，我们将进行全基因组关联分析，以直接确定商品猪群体中滴水损失的致病基因座。选定的候选基因将被详细表征，以提供标记，用于改善肉类品质方面的育种计划，理想情况下还可以识别致病突变本身。

In this project we will investigate the genes underlying drip loss variation in commercial pig populations. On the one hand, we will continue to analyse candidate genes identified during the initial phase of the DRIP research unit. On the other hand, we will also employ novel cutting edge experimental strategies, which became only available during the last two years. We plan to study the expression of all transcripts including non-coding RNAs in selected regions of the QTL-intervals using tiling microarrays. With this approach we will look for differences in the expression patterns between phenotypically divergent and QTL-genotype divergent pigs. Furthermore, we will perform a genome-wide association analysis to directly identify the causative loci for drip loss in commercial pig populations. Selected candidate genes will be characterised in detail to provide markers for improved breeding programs with respect to meat quality and ideally also to identify the causative mutations themselves.