New Synthetic Methods for Building Chip-Based Libraries

该项目专注于开发基于芯片的可寻址微阵列中电极旁的分子的现场选择性制备所需的化学开发。将进一步研究在上一个资助期制定的试剂合成/限制策略、在微阵列中选择性地使用Lewis酸以及构建核心分子支架的策略。除了这些合成努力外，还将研究用于将小分子附着到微阵列表面的新聚合物和连接物，以及微阵列用于确定添加了生物分子的库中分子的相对结合常数的用途。通过这一奖项，有机和高分子化学项目支持了华盛顿大学化学系教授凯文·D·默勒的研究。S教授的研究工作围绕芯片可寻址微阵列中分子文库(目标化合物的集合)的高效制备展开。这种格式的文库在生物医学研究中有重要的应用，因为它们可以用生物系统产生的微量材料来识别目标基因产物。以这种微阵列形式制备文库的能力对于开发具有新特性的材料也很重要。

This project is focused on the development of chemistry needed for the site-selective preparation of molecules next to the electrodes in chip-based, addressable microarrays. Further studies of the reagent synthesis/confinement strategies developed in the previous funding period, site-selective use of Lewis acids in microarrays, and strategies to construct core molecular scaffolds will be performed. In addition to these synthetic efforts, new polymers and linkers for attaching small molecules to the surface of a microarray, utility of the microarray for determining relative binding constants for the molecules in a library with an added biomolecule will also be investigated. With this award, the Organic and Macromolecular Chemistry Program is supporting the research of Professor Kevin D. Moeller of the Department of Chemistry at Washington University. Professor Moeller s research efforts revolve around the efficient preparation of molecular libraries (collection of target compounds) in chip-based, addressable microarrays. Libraries in such format have significant applications in biomedical research, as they could be used to identify target gene products with the tiny amounts of material generated by biological systems. Ability to prepare libraries in such microarray format is also important for developing materials with novel properties.