Acquisition of a 600 MHz Cryogenic Probe for Research and Education for the NMR Facility at the City College of New York

该奖项是为了表彰该学院的磁共振设备中安装的600 MHz核磁共振光谱仪的低温探头。与传统的三谐振探头相比，低温探头的灵敏度将提高3-4倍。此外，由于13C增强，它将极大地提高13C直接检测的能力，其灵敏度与专用13C检测探针相当，从而允许开发和实施更多的生物分子实验方法。此外，新的耐盐设计将防止在高盐条件下灵敏度增益的显著下降，而高盐条件通常是稳定溶液中生物分子所必需的。这一探头的可用性将使无法在高浓度下产生的样品的数据获取和/或分析速度更快。该探针将有助于广泛的研究领域，包括(I)发展新的核磁共振方法来研究生物分子结构和动力学，(Ii)确定黄素蛋白的结构-功能关系，(Iii)研究有序和无序蛋白质中的电荷效应，以及(Iv)阐明膜蛋白水解酶功能的分子决定因素。纽约城市学院是一所为少数族裔服务的机构。在核磁共振设施中提供低温探测器将使更多的学生(研究生和本科生)和博士后研究人员得到培训，包括那些属于科学界代表性不足的社区的人。有计划开发一个核磁共振研讨会，以弥合理论和“动手”实验之间的差距。这对大学生和整个州的结构生物学社区都是有益的。

This award is for the acquisition of a cryogenic probe for a 600 MHz NMR spectrometer housed in the Magnetic Resonance Facility at the college. The cryogenic probe will afford a 3-4-fold enhancement in sensitivity compared with conventional triple-resonance probes. In addition, being "13C-enhanced", it will greatly improve capabilities for 13C direct detection with sensitivities comparable to dedicated 13C-detect probes, allowing additional experimental methodology for biomolecules to be developed and implemented. Further, the new salt-tolerant design will prevent a significant degradation of the sensitivity gain in high-salt conditions that are often necessary to stabilize biomolecules in solution. The availability of this probe will allow faster data acquisition and/or analysis of samples that cannot be produced at high concentrations. The probe will benefit a broad spectrum of research areas including (i) the development of novel NMR methodology to study biomolecular structure and dynamics, (ii) determination of structure-function relationships in flavoproteins, (iii) investigation of charge effects in ordered and disordered proteins and (iv) the elucidation of the molecular determinants of membrane protease function. The City College of New York is a minority-serving institution. The availability of the cryogenic probe at the NMR facility will allow the training of a larger number of students (graduate and undergraduate) and postdoctoral researchers, including those belonging to communities that are underrepresented in the sciences. There are plans to develop an NMR workshop that would bridge the gap between theory and "hands-on" experimentation. This would be beneficial both for the college students and for the state's structural biology community in general.