Enhanced Performance Membranes by High Throughput Modification

提案标题：通过高产能改性增强性能膜提案编号：CBET-0730449主要研究者：Georges Belfort机构：Rensselaer Polytechnic Institute该项目的主要目标是开发一种快速，高效，可重复的高通量平台（HTP）修饰方法，采用光诱导接枝聚合（PGP），将允许合成，评估，以及从数百种功能化表面中快速选择最佳聚合物。 这项工作也将提供见解，在分析接枝的机制，以指导设计的表面膜分离。 在生物技术、食品和废水处理工业中需要新的、低生物污染的合成膜。需要表面或功能特性来减少膜蛋白质污染的科学基础主要是经验性的。 本研究的四个具体目标是：1。使用高通量96膜孔格式将大量市售乙烯基和胺单体光接枝到聚醚砜PES膜上。2.使用相同的高通量96膜孔格式，通过单溶质过滤检测和筛选来自目标1的性能最佳的接枝聚合膜。3.使用高通量96膜孔格式来优化和微调模型蛋白和三种相关生物技术进料的接枝和过滤条件。4.从结构-性质关系建模以及结构、动力学和结合研究中研究成功单体能够表现最佳的潜在机制原因。 拟议的研究将对上述行业的生化过程产生广泛影响。一种新的HTP方法用于材料开发和阐明接枝蛋白质抗性层的一些主要要求将是重要的成果。将寻求用于接枝PES的最佳单体，其在单一蛋白质进料的情况下表现出最佳性能。 三个最相关的生物技术饲料，E。大肠杆菌肉汤、中国仓鼠卵巢细胞培养物和牛血清。 从事该项目的研究生和本科生将接触现代高通量技术，界面科学，分析化学，相关分析和生物加工。每年高中毕业生将参观PI的实验室，对这项研究的基本方面进行评估。

Proposal Title: Enhanced Performance Membranes by High Throughput ModificationProposal Number: CBET-0730449Principal Investigator: Georges BelfortInstitution: Rensselaer Polytechnic InstituteThe main objective of this project is to develop a fast, efficient, and reproducible high throughput platform (HTP) modification method with photoinduced graft polymerization (PGP) that will allow synthesis, evaluation, and quick selection of the best polymer from many hundreds of functionalized surfaces. This work will also provide insights in analyzing the mechanism of grafting to guide design of surfaces for membrane separations. There is a need in the biotechnology, food, and wastewater treatment industries for new, low biofouling synthetic membranes. The scientific basis, for which surface or functional characteristics are needed to reduce membrane protein fouling, is largely empirical. The four specific aims for this research are: 1. To use a high throughput 96 membrane-well format for photo-grafting a large library of available commercial vinyl and amine monomers onto poly ether sulfone PES membranes. 2. To use the same high throughput 96 membrane-well format to test and screen for the best performing graft polymerized membranes from Aim 1 using single-solute filtration. 3. To use a high throughput 96 membrane-well format to optimize and fine-tune the grafting and filtration conditions for model proteins and three relevant biotechnology feeds. 4. To investigate from structure-property relationship modeling and from structural, kinetic and binding studies, the underlying mechanistic reasons why the successful monomers were able to perform best. The proposed study will have broad impact with respect to biochemical processes in the industries mentioned above. A new HTP method for materials development and elucidating some of the major requirements for a grafted protein resistant layer will be important outcomes. The best monomers for grafting PES that exhibit optimal performance with single protein feeds will be sought. Three of the most relevant biotechnology feeds, E. coli broth, Chinese hamster ovary cell culture, and bovine serum, will be used. Graduate and undergraduate students working on this project will be exposed to modern high throughput technology, interfacial science, analytical chemistry, correlation analysis and bioprocessing. Each year high school seniors will visit the PI's laboratory for a evaluation of the essential aspects of this research.