Multilibrary Strategies for Developing Complex Transgenic Microbial Phenotypes

CBET-0756451 Papoutsakis智力优点该项目旨在开发新的基于多文库的方法，用于产生复杂的表型，这将有助于开发工业上重要的菌株，用于许多生物加工应用，例如目前在生物加工中使用或预期的可再生资源利用（绿色技术）或生物修复。此外，该项目产生的知识将对乙醇耐受性的基础生物学做出重大贡献。更广泛的影响：这个项目？本发明的策略可应用于任何期望的生物体和生物加工应用，其中可选择的复杂表型是期望的，特别是用于利用可再生资源的方法。过去几个月，高效且经济可行的技术对于从可再生资源生产化学品生物燃料的重要性已成为国家和国际的紧迫优先事项。这是由几个重要事实驱动的。首先，石油等不可再生能源的枯竭，加上快速发展的经济体和世界人口推动的能源需求增加，导致石油和能源价格高涨。第二个重要的事实是认识到不可再生的化石燃料导致重大的气候变化，可能导致灾难性的天气模式变化。相比之下，生物质是用于生产生物燃料和化学品的碳中性可再生资源。最后，该项目为学生提供了实验基因组学和相关高通量技术的教育和培训的独特机会。

CBET-0756451PapoutsakisIntellectual Merit This project aims to develop novel multi-library-based approaches for generating complex phenotypes that would be useful for developing industrially important strains for many bioprocessing applications, such as those currently employed or anticipated in the bioprocessing for the utilization or renewable resources (green technologies) or in bioremediation. In addition, the knowledge generated from this project will make a significant contribution to the basic biology of ethanol tolerance. Broader Impact: This project?s strategies can be applied to any desirable organism and bioprocessing application whereby a selectable complex phenotype is desirable, and especially processes for the utilization of renewable resources. The importance of efficient and economically viable technologies for production of chemicals & biofuels from renewable resources has become an urgent national and international priority over the last few months. This was driven by several important facts. First, the depletion of non-renewable energy sources, such as oil, coupled with increased energy demand driven by fast developing economies and world population has led to high oil and energy prices. And a second important fact is the realization that non-renewable fossil fuels lead to significant climatic changes that may lead to catastrophic weather-pattern changes. In contrast, biomass is a carbon-neutral renewable resource for production of biofuels and chemicals. Finally, this project provides unique opportunities for student education and training in experimental genomics, and associated high-throughput technologies.