MRI: Acquisition of a multiphoton confocal microscope for research, training and teaching

该多光子激光扫描共聚焦显微镜的采购合同建立了一个共享使用的成像设施，将为洛马林达大学的基础科学家提供各种广泛的研究和培训。这种先进的显微镜系统将提供广泛的成像技术，包括多光子和光谱分析，以及实时成像。该系统配备了用于可见光和多光子激发的紫外和多激光器，用于多光谱探测和点扫描的多通道扫描模块，一个透射光探测器和2个用于多光子成像的非扫描探测器。这种激发和发射探测系统阵列将为研究人员提供使用多个荧光团进行创新多维成像研究的能力。为了促进对细胞相互作用和功能的基本机制的研究，并进行活细胞和组织的研究，该系统将包括孵育阶段、电生理系统和肌图仪。共聚焦显微镜将被整合到显微镜培训计划、学术课程和培训研讨会中。所提出的系统所获得的图像的视觉性质为学生从事研究提供了一个极好的切入点。学生将通过洛马琳达大学（Loma Linda University）已建立的外联、研究实习和研究生项目招收，这些项目的目标是高中生、本科生、研究生和研究生学员，重点是少数族裔。我们使用拟议仪器的目标是促进洛马林达大学和周边社区的多学科研究和培训。该设施及其成员将提供资源基础和必要的基础设施，以满足持久合作和教育的需求，并作为促进多样化学术界基础科学生产力的催化剂。

This award for the acquisition of a multiphoton laser scanning confocal microscope has established a shared-use imaging facility that will serve varied and extensive research and training purposes for basic scientists at Loma Linda University. This advanced microscopy system will provide a broad range of imaging technologies including multiphoton and spectral analysis, as well as real-time imaging. The system is equipped with UV and multiple lasers for visible and multiphoton excitation, a multi-channel scanning module for multispectral detection and point scanning, a transmitted-light detector, and 2 non-descanned detectors for multiphoton imaging. This array of excitation and emission detection systems will provide researchers the capacity to use multiple fluorophores for innovative multidimensional imaging studies. To promote investigation of basic mechanisms of cellular interactions and function and perform live-cell and tissue studies, the system will include an incubation stage, an electrophysiology system, and myograph apparatus. The confocal microscope will be integrated into a microscopy training program, academic courses, and training workshops. The visual nature of the images acquired by the proposed system make an excellent entry point for students to engage in research. Students will be recruited through established outreach and research internship and graduate programs at Loma Linda University that target high-school age, undergraduate and graduate students and post-graduate trainees with a strong emphasis on under-represented minorities. Our goal for use of the proposed instrumentation is to facilitate multidisciplinary research and training at Loma Linda University and in the surrounding community. This facility and its members will provide the resource base and essential infrastructure that meet the needs for enduring collaboration and education, as well as serve as a catalyst that promotes basic science productivity in a diverse academic community.