GOALI: 2D Optoelectronic Graphene Nanoprobes for Neural Network

1264982-徐本研究的目标是通过一个独立的二维光电石墨烯平台，从亚微米到厘米尺度，研究从单突触到多神经元电路的信号处理原理。该方法是直接在大面积悬浮的石墨烯薄膜上培养神经元，这是液体环境中超灵敏石墨烯晶体管的一部分。通过石墨烯光导测量、膜片钳记录和光学成像，将研究穿过神经元电路的轴突、树突和突触的电、化学和光学变化。概述：神经元活动通常使用电极阵列来绘制，该电极阵列刺激神经细胞并记录其电反应。这个系统有几个局限性：电极的数量通常很少，电池相对于电极的位置没有很好地确定。这项提议将使用新型材料--石墨烯，作为培养神经元的表面。石墨烯具有光电效应，这意味着细胞的电活动可以用显微镜进行光学监测。这将绕过电极阵列的限制，并允许监测更多数量的神经细胞的活动。

1264982 - XuThe objective of this research is to investigate, across submicron to centimeter size scales, the principle of signal processing from single synapse to multi-neuron circuitry, via a 2D free-standing optoelectronic graphene platform. The approach is to grow neurons directly on a large-area suspended graphene membrane, which is part of an ultra-sensitive graphene transistor in a liquid environment. Through graphene photoconductance measuring, patch-clamp recording, and optical imaging, the electrical, chemical, and optical changes in axons, dendrites and synapses across a neuronal circuit will be investigated.General statement:Neuronal activity is typically mapped using electrode arrays that stimulate neuronal cells and record their electrical responses. This system has several limitations: the number of electrodes is typically small and placement of cells in relation to electrodes is not well defined. This proposal will deploy novel material, grapheme, to be used as the surface for culturing neurons. Grapheme has optoelectrical effect, meaning that electrical activity of cells may be monitored optically using a microscope. This would circumvent limitations of electrode arrays and would allow for monitoring activity of a much larger number of neuronal cells.