Molecular effects of cold atmospheric plasma on tumor cells

在过去的几年里，冷大气等离子体(CAP)在医学上的应用已经发展成为一个迅速增长的重要的创新研究领域。CAP的一个很有前途的新医学应用是癌症治疗。不同的研究表明，CAP可能潜在地影响细胞周期，并导致肿瘤细胞的细胞凋亡或坏死，这取决于CAP设备和剂量。该小组的数据显示，一种新开发的等离子体设备利用表面微放电(SMD)技术在空气中产生等离子体对黑色素瘤和其他类型的肿瘤有很强的效果。在这项研究中，我们将使用新的CAP设备，并因此分析CAP对癌细胞(主要是黑色素瘤)的剂量依赖性治疗效果。我们的目标是通过分析信号通路、转录调控和各自的靶基因来了解CAP对肿瘤细胞影响的分子基础。此外，正常细胞和血浆的假定作用也将通过类似的方法确定。利用这些知识建立了一个基本的理解，可以应用于肿瘤治疗，并进一步用于支持肿瘤特异性血浆的设计。

Over the past few years, the application of cold atmospheric plasma (CAP) in medicine has developed into an innovative field of research of rapidly growing importance. One promising new medical application of CAP is cancer treatment. Different studies revealed that CAP may potentially affect the cell cycle and cause cell apoptosis or necrosis in tumor cells dependent on the CAP device and doses. Data of the group revealed strong effects of a newly developed plasma device utilizing the Surface Micro Discharge (SMD) technology for plasma production in air on melanoma and other tumor types. In this study we will used the novel CAP device and consequently analyzed doses-dependent CAP treatment effects on cancer cells, mainly melanoma. We aim to understand the molecular basis of CAP effects on tumor cells by analyzing signaling pathways, transcriptional regulation and respective target genes. In addition, normal cells and the putative action of plasma will be determined by similar means. Using this knowledge a basic understanding is created which can be applied in tumor therapy and further be used to support the design of tumor-specific plasma.