In situ cross-linking hydrogels for regeneration of cartilage

原位交联水凝胶可用于组织缺损的微创治疗，也可用于药物的控制释放。基于纤维素和壳聚糖的半合成硫化和氧化多糖可用于制备具有可调节的机械性能、降解行为、生长因子转化生长因子-β3的释放动力学以及对间充质干细胞的软骨形成活性的水凝胶，从而适用于软骨再生的假设。除了纤维素和壳聚糖衍生物的合成、机械降解性能的表征和生物活性的体外研究外，还应利用光谱和成像方法分析水凝胶内的传质、力学性能和生物相容性，并建立小鼠模型。

In situ cross-linking hydrogels are useful for minimal invasive treatment of tissue defects and also for controlled release of drugs. The proposal is focused on the hypothesis that semisynthetic sulfated and oxidized polysaccharides based on cellulose and chitosan can be used for generation of hydrogels with tunable mechanical properties, degradation behavior, kinetic of release of growth factor TGF-beta 3 and resulting chondrogenic activity towards mesenchymal stem cells to be applicable for regeneration of cartilage. Beside synthesis of cellulose and chitosan derivatives, characterization of mechanical degradation properties and in vitro studies on bioactivity, also spectroscopic and imaging methods shall be used to analyze mass transfer inside hydrogels, mechanical properties and biocompatibility in vitro and in vivo with a mouse model.