Functional analysis of Symbiosis Receptor Kinase (SYMRK) mediated signal perception

共生受体激酶(SYMRK)是植物与AM真菌、放线菌宿主与Frankia细菌、豆科植物与根瘤菌之间成功共生的必要条件。SYMRK是一种膜结合型受体样激酶(RLK)，在胞外区和胞内区各有3个富含亮氨酸的重复序列。这个项目解决的问题是SYMRK如何调节植物细胞的发育重编程，以响应内共生微生物，如丛枝菌根(AM)真菌或固氮根瘤菌。在第一个资助期，我们已经建立了重要的工具，用于在植物中检测SYMRK，并鉴定了与SYMRK相互作用的内源蛋白。我们现在将使用反向遗传学来分析它们的功能相关性。此外，我们的目标是辨别SYMRK复合体的亚细胞和组织定位，以及共生信号分子和/或相互作用组分如何影响SYMRK的时空动力学。我们希望这个项目能为共生信号机制和植物受体蛋白的功能提供新的见解。

The symbiosis receptor kinase (SYMRK) is required for successful symbiotic interactions between plants and AM fungi, between actinorhiza hosts and Frankia bacteria and between legumes and rhizobia. SYMRK is a membrane bound receptor-like kinase (RLK) with 3 leucine-rich repeat motifs in the extracellular domain and an intracellular protein kinase domain. This project addresses the question how SYMRK mediates developmental reprogramming of plant cells in response to endosymbiotic microorganisms such as arbuscular mycorrhiza (AM) fungi or nitrogen-fixing rhizobia. During the first funding period we have established important tools for the biochemical and microscopical detection of SYMRK in planta and identified endogenous proteins interacting with SYMRK. We will now analyse their functional relevance using reverse genetics. Moreover we aim to discern the subcellular and tissue localization of SYMRK complexes and how symbiotic signalling molecules and/or interactome components influence the spatiotemporal dynamics of SYMRK. We expect this project to provide novel insights into symbiotic signalling mechanisms and plant receptor kinase function in general.