Elucidation of malaria erythrocyte invasion system using stem cell techonology

我们用人脐血中的红细胞样细胞、造血干细胞和小鼠的ES细胞证实了恶性疟原虫的入侵。干细胞转化为成熟红细胞的有效生产体系的建立已基本完成。在ES细胞和iPS细胞的治疗前，已经掌握了从脐血中分离培养CD34阳性造血干细胞的技术。在此基础上，从细胞因子种类、细胞因子浓度、培养周期等方面探讨了诱导去核的有效条件，并利用慢病毒载体建立了分化红细胞转导系统，以获得特异性蛋白缺失的分化红细胞突变体。

We confirmed the invasion of Plasmodium falciparum using erythrocyte like cells from human umbilical cord blood hematopoietic stem cell and mouse ES cells. The establishment of effective production system of mature erythrocyte from stem cell has been mainly proceeded. Before the treatment of ES cell and iPS cell, the technique that CD34 positive hematopoietic stem cell are isolated from umbilical cord blood and cultured have been mastered. Afterwards, we investigated the effective condition to lead enucleation with arrangement of class, concentration of cytokine, and culture period.Moreover, to produce the differentiated erythrocyte mutant with specific protein deficient, we tried to establish the trancfection system of differentiated erythrocyte using lentivirus vecter.