Generation of transgenic mouse for analysis of in vivo dynamics of influenza virus infection

为了获得能广泛表达编码GFP基因的重组流感病毒样RNA的转基因小鼠，我们构建了重组RNA的表达载体。用所构建的质粒预先转染培养的细胞，然后感染野生型流感病毒，在感染病毒的细胞中特异性地表达GFP。将该基因的转录区片段注入小鼠受精卵中，获得了3个基因阳性的小鼠品系。然而，即使在感染野生型流感病毒后，这些动物或它们的尾部来源的原代细胞也不表达GFP。这些结果表明，为了完成这项研究，应该对该质粒的转录区域进行修饰。

To generate transgenic mouse ubiquitously expressing a recombinant influenza virus-like RNA encoding GFP gene, we constructed a plasmid for the expression of the recombinant RNA. Cultured cells pre-transfected with the constructed plasmid and subsequently infected with wild-type influenza viruses expressed GFP specifically in virus-infected cells. By injecting the transcriptional region fragment of the plasmid into mouse fertilized eggs, we obtained three mouse lines whose germlines were positive for the injected genes. The animals or their tail-derived primary cells, however, did not express GFP even after infection with wild-type influenza viruses. These results imply that the transcriptional region of the plasmid should be modified to complete this study.